Background: The implementation and usage of digital pathology has undergone a huge development in recent years however the area of intraoperative consultation has not yet become digitized or fully investigated. The aim of this study was to explore the possibilities to digitize this area and consistency between the diagnoses based on the digital slides versus the traditional microscope. The whole slide image scanner Olympus VS200 STL proclaimed that it would be able to scan glass slides with wet mounting glue. Method: To find the optimal scanning profile a field of four different scan profiles was tested in different contexts. The chosen profile was used to generate 126 digital slides from 60 cases. These slides were assessed on three parameters; compliance between diagnosis of the digital and traditional method, compliance with the visual quality and could the scan time including operating time be completed within 180 seconds pr slide. Results: The overall result showed no deviation between diagnoses made with conventional microscope and the digital slide in 83% of the cases and the average operation time were 92.5 seconds. Conclusion: Olympus VS200 STL has the potential to become implemented in a clinical pathology department for use in intraoperative diagnostics without affecting workflow, diagnostic accuracy, and acceptable timeframe (180 seconds pr slide).
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