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The anaerobically-regulated nirB promoter was used to express heterologous genes in Escherichia coli. Under anaerobic conditions the promoter was able to express tetanus toxin fragment C at approximately 20% total cell protein (tcp) and the Bordetella pertussis antigen pertactin at greater than 30% tcp. These levels are comparable to those obtained for the same products using the tac promoter. The nirB promoter is very well regulated, giving almost two orders of magnitude increase in fragment C on complete removal of oxygen.

Patients have many symptoms that are not SR on admission. SQ plays a vital role in the detection of symptoms that may require further assessment or treatment.

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It is recognized that empowering individuals to manage their own health and wellbeing will result in more cost-effective healthcare systems, improved health outcomes and will encourage healthy individuals to remain that way. With the advent of the quantifiedself movement in recent years, there has been an increase in technology applications supporting wellness self-management. Such applications allow people to self-track and self-report, with many providing feedback. However, little research in this area has examined how best to support older adults in health selfmanagement. This paper reports findings from a 5-month home deployment of YourWellness -an application that supports older adults in self-reporting on their wellbeing and provides feedback to promote positive wellbeing management. Our findings contribute to a greater understanding of older adults' attitudes and behaviours in relation to wellbeing self-management that can facilitate the creation of new, personalized health and wellbeing interventions for this population.

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In community-dwelling older adults, these preliminary results show that a cognitive dual-task significantly (P < 0.025) affects QTUG performance in almost all parameters, with a significant (P < 0.025) reduction in time-to-stand observed with a motor task. Although no statistical difference was found between fallers and non-fallers for many of the parameters, cadence, stride time and stride velocity were statistically different (P < 0.05). A larger sample size and more assessment points might lead to more definitive findings. These results highlight the need for further research to examine QTUG performance under dual-task conditions between fallers and non-fallers in this population, and to look at the ability of dual-task QTUG assessment to measure change longitudinally and the effectiveness of therapeutic interventions. Geriatr Gerontol Int 2017; 17: 1176-1182.