714 RESUMOContrastes radiológicos iodados -CI são causa de lesão renal aguda -LRA. Avaliar o efeito renoprotetor do bicarbonato de sódio (Bic) sobre a função renal (clearance de crea nina, Jaff é, Clcr-ml/min/100g) e o perfi l oxida vo (excreção de peróxidos, PU e de malondealdeído urinários, FOX-2 e TBARs, nmol/mgCr ) em ratos com CI. Ratos machos adultos Wistar, 250-300g, tratados 1x/dia, por 5 dias, foram divididos nos grupos: Salina (solução salina 0,9%, 3ml/kg/dia, intraperitoneal-i.p.); CI (ioxitalamato de meglumina e sódio, 3ml/kg, i.p); Bic+Salina (Bic 3ml/kg, i.p, 1 hora antes e 1 hora depois da Salina); Bic+CI (Bic 3ml/ kg, i.p, 1 hora antes e 1 hora depois do CI). DESCRITORES ABSTRACTRadiological iodinated contrasts (IC) agents cause acute kidney injury (AKI). To evaluate the renoprotec ve eff ect of sodium bicarbonate (Bic) on renal func on (crea nine clearance [Clcr], Jaff é, and Clcr mL·min -1 100 g -1 ) and the oxida ve profi le (peroxide excreon, urinary peroxides, urinary malondialdehyde, FOX-2 expression, and thiobarbituric acid reactive substance [TBARS; nmol/mg Cr]) in rats treated with an IC agent. Adult male Wistar rats weighing 250-300 g were treated once daily for 5 days with one of the following treatments: saline (0.9%, 3 mL·kg -1 day -1 intraperitoneally [i.p.]), IC agent (sodium and meglumine ioxitalamate, 3 mL/kg, i.p.), Bic + Saline (3-mL/kg Bic, i.p., 1 h before and a er saline treatment), and Bic + IC (3-mL/kg Bic, i.p., 1 h before and a er the IC treatment). The IC agent induced AKI, and the antioxidant renoprotective effect of Bic was confirmed (Clcr/TBARS/urinary peroxide: saline group, 0.59 ± 0.03/0.11 ± 0.02/1.29 ± 0.24; Bic + Saline group, 0.58 ± 0.03/0.13 ± 0.02/1.32 ± 0.64; IC group, 0.22 ± 0.02/0.19 ± 0.02/4.77 ± 0.24; Bic + CI group, 0.51 ± 0.04/0.13 ± 0.3/1.80 ± 0.04; p<0.05). The protec ve eff ect of Bic in the ICinduced AKI was confi rmed; hence, Bic administra on may be considered as a therapeu c op on for pa ents undergoing IC-enhanced radiography.
Objective: To investigate the renoprotective effects of the extract of Vitis vinifera L in the Tacrolimus-induced nephrotoxicity in experimental studies with rats. Methods: Quantitative survey with an experimental model in which adult male, Wistar rats, weighing between 250g -300g, were used. The rats were treated once a day for cincodias by gavage as follows: Saline (control, sodium chloride, 0.1 ml); Vitis (Vitis vinifera L 3mg/kg), FK (0.5 mg / kg); and, FK + Vitis (0.5 mg / kg + 3 mg / kg, respectively). Renal function was assessed by creatinine clearance (CrCL / 100g, Jaffe method) and lipid peroxidation by measurement of urinary peroxides (PU, FOX-2). Results: The administration of FK increased the excretion of peroxides and reduced creatinine clearance, and simultaneous administration with Vitis vinifera L protected the renal function in these parameters. Conclusion: These data confirm the injury induced by nephrotoxic Tacrolimus and demonstrated the renoprotective effect of Vitis vinifera L. Keywords: Acute kidney injury/chemically induced; Immunosuppressive agents/toxicity; Vitis vinifera L ; Rats, Wistar RESUMOObjetivo: Verificar o efeito renoprotetor do extrato de Vitis vinifera L na nefrotoxicidade induzida pelo Tacrolimus em estudos experimentais com ratos. Métodos: Pesquisa quantitativa com modelo experimental, na qual foram utilizados ratos Wistar, machos, adultos, pesando entre 250g -300g, tratados uma vez ao dia por cincodias, por gavagem, conforme segue: Salina (controle, cloreto de sódio 0,1ml); Vitis (Vitis vinifera L 3mg/kg), FK (0,5mg/kg) e FK+Vitis (0,5mg/kg + 3mg/kg, respectivamente) . A função renal foi avaliada por meio do clearance de creatinina (Clcr/ 100g, método Jaffé) e a peroxidação lipídica pela mensuração de peróxidos urinários (PU, FOX-2). Resultados: A administração de FK elevou a excreção de peróxidos e reduziu o clearance de creatinina, e a administração simultânea com Vitis vinifera L protegeu a função renal nesses parâmetros. Conclusão: Os dados confirmaram a lesão nefrotóxica induzida pelo Tacrolimus e demonstraram o efeito renoprotetor do Vitis Vinifera L. Descritores: Lesão renal aguda/induzido quimicamente; Imunossupressores/toxicidade; Vitis vinifera L; Ratos Wistar RESUMEN Objetivo: Verificar el efecto renoprotector del extracto de Vitis vinifera L en la nefrotoxicidad inducida por el Tacrolimus en estudios experimentales con ratas. Métodos: Investigación cuantitativa con modelo experimental, en la cual fueron utilizadas ratas Wistar, machos, adultos, que pesaban entre 250g -300g, tratados una vez al dia por cinco días, por alimentación forzada, conforme sigue: Salina (control, cloreto de sodio 0,1ml); Vitis (Vitis vinifera L 3mg/kg), FK (0,5mg/kg) e FK+Vitis (0,5mg/kg + 3mg/kg, respectivamente) . La función renal fue evaluada por medio del clearance de creatinina (Clcr/ 100g, método Jaffé) y la peroxidación lipídica por la mensuración de peróxidos urinarios (PU, FOX-2). Resultados: La administración de FK elevó la excreción de peróxidos y redujo el clearance...
Our findings highlight the importance for the development of quality initiatives in identifying gaps and weaknesses in the process that should be corrected or even restructured, therefore maximizing the number of donors and organs transplanted. Hospitals that participate in the NOP process met 61% of the quality indicators proposed by ODEQUS. Identification of potential donors, family consent, conversion, and sudden cardiac arrest rates are areas that did not conform to ODEQUS standard and revealed a great opportunity for improvement.
Objective To understand the profile of professionals working in organ harvesting, and analyze the learning results of those trained before and after the course on recovery, perfusion and packaging of organs for transplants.Methods A retroprospective, quantitative, analytical-descriptive study about the Course on Recovery, Perfusion and Packaging of Liver and Kidney, in the period from 2012 to 2014. Pre- and post-tests, with ten questions were used to assess knowledge about organ harvesting. The association of knowledge with applied content was verified by the McNemar test.Results Of the total of 334 participants, 187 (56.0%) were physicians, 104 (31.1%) nurses, and 43 (12.9%) scrub nurses. The majority of participants was male (58.4%), mean age of 39.1 years, 50% had graduated 5 to 10 years before, and 50.4% had less than one-year experience in organ harvesting. In knowledge assessment, there was an increase in the weighted mean, from 6.1 in the pre-test to 7.9 in the post-test. A significant increase in learning was observed in the post-test in 50% of scrub nurses, 33.3% in nurses 20% in physicians.Conclusion The professionals were starting work in organ harvesting, and most were from Southeastern, Northeastern and Northern regions. In terms of learning, the course contributed to enhancing knowledge of the multiprofessional health team, and represented better learning standard.
Introdução: Identificar a fidedignidade dos critérios para a abertura do protocolo de morte encefálica, conforme estabelecido na Resolução do Conselho Federal de Medicina (CFM) 1.480/97; caracterizar o perfil dos potencias doadores. Método: Trata-se de um estudo retrospectivo com abordagem quantitativa, realizado em um hospital municipal de grande porte da região Sul, na cidade de São Paulo. Foram selecionados os prontuários de pacientes diagnosticados com morte encefálica durante o período de 1 de janeiro a 30 de junho de 2013. O estudo foi aprovado pelo Comitê de Ética em Pesquisa, sob o CAAE 20387913.0.0000.5452. Resultados: Foram diagnosticados 16 pacientes com morte encefálica. O tempo entre os exames clínicos variou de seis a 30 horas. Nenhum paciente apresentou Pressão Arterial Média < 60 mmHg. Os sedativos utilizados pelos pacientes foram Fentanil, Midazolam e Thiopental, sendo que, em todos os protocolos, foi respeitado o tempo de suspensão dessas drogas para a abertura dos mesmos. Em apenas um protocolo o potencial doador apresentou temperatura corpórea < que 35 oC, tanto na primeira quanto na segunda avaliação clínica, 34,5 oC e 33,3 oC, respectivamente. Quanto à realização do teste de apneia, foi utilizada a gasometria arterial pós-apneia em 13 casos no primeiro exame clínico e em 14 no segundo, sendo que nos demais a avaliação foi observacional, isto é , visualização direta do tórax quanto à presença de movimentos respiratórios. Conclusão: Em todos os protocolos de morte encefálica foram respeitados os critérios exigidos para a abertura do mesmo, de acordo com a Resolução do Conselho Federal de Medicina 1.480/97.
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