In
this paper, we describe the elucidation of the target of an
aptamer against ovarian cancer previously obtained by cell-SELEX (SELEX
= systematic evolution of ligands by exponential enrichment). The
target’s identity, stress-induced phosphoprotein 1 (STIP1),
was determined by mass spectrometry and validated by flow cytometry,
using siRNA silencing and protein blotting. Initial oncologic studies
show that the aptamer inhibits cell invasion, indicating that STIP1,
which is currently under investigation as a potential biomarker for
ovarian cancer, plays a critical role in this process. These results
serve as an excellent example of how protein target identification
of aptamers obtained by cell-SELEX can serve as a means to identify
promising biomarker candidates and can promote the development of
aptamers as a new drug class to block important oncological processes.
openAccessArticle: Falsecover date: 2015-10-01pii: S0196-0644(15)00773-8Harvest Date: 2016-01-06 13:08:22issueName: Research Forum Abstracts 2015Page Range: S54-S54href scidir: http://www.sciencedirect.com/science/article/pii/S0196064415007738pubType: Research forum abstrac
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