Segmentations are often necessary for the analysis of image data. They are used to identify different objects, for example cell nuclei, mitochondria, or complete cells in microscopic images. There might be features in the data, that cannot be detected by segmentation approaches directly, because they are not characterized by their texture of boundaries, which are properties most segmentation techniques rely on, but morphologically. In this report we will introduce our algorithm for the extraction of suchlike morphological features of segmented objects from segmentations of neuromuscular junctions and its interface for informed parameter tuning.
3D segmentation has become a widely used technique. However, automatic segmentation does not deliver high accuracy in optically dense images and manual segmentation lowers the throughput drastically. Therefore, we present a workflow for 3D segmentation being able to forecast segments based on a user-given ground truth. We provide the possibility to correct wrong forecasts and to repeatedly insert ground truth in the process. Our aim is to combine automated and manual segmentation and therefore to improve accuracy by a tunable amount of manual input.
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