We evaluated in vitro the influence of nickel‐titanium instruments kinematics on the accuracy and variation of root canal working length measurements, performed with an integrated apex locator, at glide path and at the end of shaping. Forty‐four mandibular incisors, included in an alginate model, were allocated at random to two groups: reciprocating and rotary. Working length was determined at glide path stage and at the end of shaping. Measurements given by the integrated apex locator were matched with visual measurements. The apex locator accuracy was based on inter‐group comparison. The variation in working length was based on intra‐group comparison. Kinematics influenced the accuracy of measurements only after shaping (p < 0.05), and not in the glide path (p > 0.05). Rotary had values closer to the visual measurements. Diminishing of measures occurred after shaping for reciprocating (p < 0.05); and at glide‐path stage for rotary (p > 0.05). The integrated apex locator was more accurate with rotary kinematics.
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