Seeds with low vigor often have problems to establish seedlings in the field. Studies have shown beneficial effects of 24-epibrassinolide (24-EpiBL) on seed performance, including germination speed, tolerance to heat stress, and initial development of seedlings. In this study, priming with 24-EpiBL was investigated on seed lots of lettuce with different vigor levels. Two cultivars (Florence and Betty) were tested, and each cultivar was represented by three lots. Different concentrations of 24-EpiBL were studied: 10-6, 10-8, and 10-10 M. Seeds were evaluated for germination and vigor (germination first count, germination speed index, saturated salt accelerated aging [SSAA], and seedling length). Unprimed seeds and seeds primed with 24-EpiBL, in the best concentration identified during germination and vigor tests, were analyzed by the Seed Vigor Imaging System (SVIS®) software to generate vigor index and growth index values. The addition of 24-EpiBL associated with priming is efficient to improve the performance of lettuce seeds. All concentrations studied were efficient, and the best was 10-6 M.
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