FILIA is a member of the recently identified oocyte/embryo expressed gene family in eutherian mammals, which is characterized by containing an N-terminal atypical KH domain. Here we report the structure of the N-terminal fragment of FILIA (FILIA-N), which represents the first reported three-dimensional structure of a KH domain in the oocyte/embryo expressed gene family of proteins. The structure of FILIA-N revealed a unique N-terminal extension beyond the canonical KH region, which plays important roles in interaction with RNA. By co-incubation with the lysates of mice ovaries, FILIA and FILIA-N could sequester specific RNA components, supporting the critical roles of FILIA in regulation of RNA transcripts during mouse oogenesis and early embryogenesis.
The shaking table array system is composed of multiple shaking tables for seismic response simulation tests of large-span spatial structures, bridge structures, slender structures such as pipeline and aqueduct, complex structures, and so on. In the process of testing with the multiple shaking tables, the interaction between the shaking tables and specimen affects the output accuracy of the shaking tables. The characteristics and rules of the dual shaking tables-specimen interaction effects on the system performance were analyzed in this paper. In order to improve the output accuracy of the dual shaking tables, force feedback compensation was introduced into three-variable control to reduce the interaction effects. However, the measurement errors of the force in the actuator and the acceleration of the shaking tables existed in the process of force feedback compensation. In order to verify the effectiveness of force feedback compensation for interaction between the dual shaking tables and specimen, the error influences on the system performance were simulated.
Multisupport, multidimension, and nonuniform excitation seismic experiments have new requirements for shaking table array system in synchronous tracking control. Therefore, this article proposed a novel synchronous tracking strategy, differential movement synchronous tracking control (DMSTC) strategy, for double-shaking table system while taking the interaction between shaking tables and specimen into consideration. DMSTC Simulink model of the double-shaking table with specimen was established and simulations were conducted in various conditions. The results demonstrate the viability of the proposed DMSTC in that the frequency bandwidth of the double-shaking tables is expanded from 3.27 Hz to 64.57 Hz, the maximum value of differential movement synchronous error is decreased from 1.682 mm to 0.482 mm, and the maximum tracking errors of the two shaking tables decrease from 1.138 mm to 0.044 mm and from 1.030 mm to 0.497 mm, respectively.
Electro-hydraulic shaking table is an essential experimental apparatus to evaluate structural performance under actual vibration condition. The control-structure interaction (CSI) between shaking table and eccentric load has lately received considerable attention for causing the accuracy degradation of shaking table test. At present, the research gap of the influence of the eccentricity of load on the CSI makes it challenging to find the CSI effects. And an effect assessment is yet to be proposed to evaluate the CSI effects, which has impeded the development of test technology. To overcome those theoretical bottlenecks, in this research, an analytical transfer function matrix of shaking table and eccentric load is established to analyze the CSI effects. The analysis is conducted under such conditions as different mass ratio (MR), moment of inertia ratio (IR), and eccentric distance ratio (ER) conditions. Through the analysis, the role of the ER is identified, the sensitivities of the MR, IR, and ER to the transfer function matrix are revealed, and the CSI effects are found. Furthermore, a novelty effect assessment is proposed to appraise whether the CSI effects can be ignored in shaking table test. And the visualization expression of the effect assessment is obtained for convenient application.
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