Leaf senescence, the last stage of leaf development, is essential for whole-plant fitness as it marks the relocation of nutrients from senescing leaves to reproductive or other developing organs. Temporally coordinated physiological and functional changes along leaf aging are fine-tuned by a highly regulated genetic program involving multi-layered regulatory mechanisms. Long noncoding RNAs (lncRNAs) are newly emerging as hidden players in many biological processes; however, their contribution to leaf senescence has been largely unknown. Here, we performed comprehensive analyses of RNA-seq data representing all developmental stages of leaves to determine the genome-wide lncRNA landscape along leaf aging. A total of 771 lncRNAs, including 232 unannotated lncRNAs, were identified. Time-course analysis revealed 446 among 771 developmental age-related lncRNAs (AR-lncRNAs). Intriguingly, the expression of AR-lncRNAs was regulated more dynamically in senescing leaves than in growing leaves, revealing the relevant contribution of these lncRNAs to leaf senescence. Further analyses enabled us to infer the function of lncRNAs, based on their interacting miRNA or mRNA partners. We considered functionally diverse lncRNAs including antisense lncRNAs (which regulate overlapping protein-coding genes), competitive endogenous RNAs (ceRNAs; which regulate paired mRNAs using miRNAs as anchors), and mRNA-interacting lncRNAs (which affect the stability of mRNAs). Furthermore, we experimentally validated the senescence regulatory function of three novel AR-lncRNAs including one antisense lncRNA and two mRNA-interacting lncRNAs through molecular and phenotypic analyses. Our study provides a valuable resource of AR-lncRNAs and potential regulatory networks that link the function of coding mRNA and AR-lncRNAs. Together, our results reveal AR-lncRNAs as important elements in the leaf senescence process.
The Juksan weir, installed in the Yeongsan river in South Korea from 2010 to 2012, has secured sustainable water resources and helped control flooding. However, low river flow velocities due to the weir have deteriorated the quality of the river water. For natural river restoration, the water gate was opened in 2017. In this study, the three-dimensional finite difference model Visual MODFLOW was used to analyze the effects of gate opening on stream–aquifer interactions. A conceptual model was developed to simulate the stream–aquifer dynamics caused by the operation of the water gate at the Juksan weir. Groundwater data were also analyzed to determine the impacts of weir operations on groundwater quality. Our results indicate that a lower river level due to the weir opening changed the groundwater flow, which then affected the water balance. The change in groundwater flow increased the variability of the groundwater quality which had homogenized because of induced recharge after the construction of the weir. This could affect groundwater use in agricultural areas near the weir. Therefore, further groundwater monitoring and hydrodynamic analyses are required to anticipate and address any potential issues.
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