Methods based on various clathrate-forming molecules, siigar analyses in combination with glucoamylase treatment, or catalytic properties of cyclodextrins were evaluated as cyclodextrin assays. Optimum conditions for the assays were determined and, when necessary, appropriate modifications were made. The tests were made by using pure cyclodextrin standards, supplemented standards, and conversion mixtures obtained by the action of cyclodextrin glycosyltransferase on starch. The optimized methods varied considerably with respect to measurement range, relative sensitivity toward CY-, p-, and 7-cyclodextrin, and susceptibility to interferences. On the basis of data from these experiments, typical applications of individual methods or their combinations are suggested. The specificity differences of assays based on methylorange, bromocresol green, and phenolphthalein were found especially useful in the analysis of individual CDs out of their mixtures. An example of the usage of this method combination is given.
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