The tuning of the spectroscopic signatures of boron-carrying fluorescent dyes by subtle chemical modifications is achieved. In more details, we propose a new series of compounds incorporating up to three...
The main goal of this study was the investigation of the impact of several ionic liquids, commonly used as free silanol suppressors, on the retention and separation of phosphorothioate oligonucleotides. Three various stationary phases (octadecyl, octadecyl with embedded polar groups and pentafluorophenyl) as well as ionic liquids with the concentration range of 0.1-7 mM were used for this purpose. The results obtained during this study showed that the increase in concentration of ionic liquids results in increasing retention of the oligonucleotides. Such an effect was observed regardless of the stationary phase used.Moreover, elongation of the alkyl chain in the structure of ionic liquids caused an increase of antisense oligonucleotide retention factors. The results obtained during retention studies confirmed that addition of ionic liquids to the mobile phase influences antisense oligonucleotide retention in a way similar to the case of commonly used ion pair reagents such as amines. A method of oligonucleotide separation was also developed. The best selectivity was obtained for the octadecyl stationary phase since separation of mixtures of antisense oligonucleotides and their metabolites differing in sequence length was successful.It has to be pointed out that ionic liquids were used for the first time as mobile phase additives for oligonucleotide analysis. View Article Online AE 0.443 29.255 AE 0.370 36.562 AE 0.198 41.360 AE 0.468 41.623 AE 0.000 OL2 13.135 AE 0.075 15.210 AE 0.148 16.695 AE 0.223 n.a. n.a. 23.688 AE 0.000 25.679 AE 0.000 28.172 AE 0.025 35.761 AE 0.395 39.686 AE 0.171 40.000 AE 0.121 OL3 10.921 AE 0.097 13.207 AE 0.025 14.862 AE 0.000 n.a. n.a. 21.666 AE 0.050 23.933 AE 0.050 26.341 AE 0.000 34.697 AE 0.122 37.768 AE 0.025 39.426 AE 0.199 OL4 14.846 AE 1.532 15.892 AE 0.075 17.235 AE 0.050 n.a. n.a. 24.684 AE 0.025 26.743 AE 0.025 29.080 AE 0.025 37.401 AE 0.50 41.238 AE 0.050 42.336 AE 0.075 OL5 13.257 AE 0.050 15.490 AE 0.050 16.780 AE 0.050 n.a. n.a. 24.282 AE 0.050 26.357 AE 0.025 28.677 AE 0.000 37.542 AE 0.000 41.222 AE 0.025 42.983 AE 0.345 OL6 11.583 AE 0.148 13.973 AE 0.079 15.524 AE 0.000 n.a. n.a. 23.585 AE 0.050 23.986 AE 2.738 28.313 AE 0.025 37.768 AE 0.075 41.291 AE 0.025 43.263 AE 0.050 This journal isFig. 5 Chromatograms obtained for: (A) OL4 extracted from enriched serum sample, (B) separation of OL1 and its potential sequence related impurity. Chromatographic conditions: (A) Kinetex F5 stationary phase, gradient elution program: 45-60% v/v of MeOH in 10 minutes, (B) syncronis aQ stationary phase, gradient elution program: 48-55% v/v of MeOH in 10 minutes. UV detection at l ¼ 260 nm, autosampler and column temperature: 30 C, mobile phase flow rate 0.3 ml min À1 . Injection volume: 2 ml. 39108 | RSC Adv., 2019, 9, 39100-39110 This journal is
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