We describe an alternative polyethylene glycol (PEG) embedding procedure which utilizes PEG 200 for dehydration and PEG 600 for infiltration and embedding of perfusion-fixed rat liver. PEG 600 has a melting point of 22*C, enabling infiltration offixed tissue to be performed at room temperature. Sections (2 iiM) cut in a cryostat at-2OaC and immobilized in agarose were readily labeled by immunoperoxidase protocols with monoclonal antibodies to hepatocyte
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