Loop ileostomy is an effective procedure to protect downstream intestinal anastomoses. Ileostomy reversal surgery is often performed within 12 months of formation but is associated with substantial morbidity due to severe post-surgical complications. Distal ileum is deprived of enteral nutrition and rendered inactive, often becoming atrophied and fibrotic. This study aimed to investigate the microbial and morphological changes that occur in the defunctioned ileum following loop ileostomy-mediated fecal stream diversion. Functional and defunctioned ileal resection tissue was obtained at the time of loop-ileostomy closure. Intrapatient comparisons, including histological assessment of morphology and epithelial cell proliferation, were performed on paired samples using the functional limb as control. Mucosal-associated microflora was quantified via determination of 16S rRNA gene copy number using qPCR analysis. DGGE with Sanger sequencing and qPCR methods profiled microflora to genus and phylum level, respectively. Reduced villous height and proliferation confirmed atrophy of the defunctioned ileum. DGGE analysis revealed that the microflora within defunctioned ileum is less diverse and convergence between defunctioned microbiota profiles was observed. Candidate Genera, notably Clostridia and Streptococcus, reduced in relative terms in defunctioned ileum. We conclude that Ileostomy-associated nutrient deprivation results in dysbiosis and impaired intestinal renewal in the defunctioned ileum. Altered host-microbial interactions at the mucosal surface likely contribute to the deterioration in homeostasis and thus may underpin numerous postoperative complications. Strategies to sustain the microflora before reanastomosis should be investigated.

Vocal responses of kittens and mature cats were recorded in a variety of standard behavioral situations. Sonographic analysis of these responses showed similarities of responses obtained repeatedly from different cats within each recording situation. Marked differences in response patterns were noted in different recording situations. The kitten and cat vocal repertoires thus include a variety of specific responses to particular motivational or behavioral circumstances.

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Evaluates the effect of the library component of a freshman orientation program on student attitudes and library anxiety. A modified version of Bostick's Library Anxiety Scale was administered to 1,027 true freshmen enrolled in the Connections First‐Year Experience program at Utah State University in the fall of 2003. First year students showed a moderate level of library anxiety prior to their library orientation sessions. This anxiety was significantly decreased after the orientation sessions. A control group reported similar anxiety levels on the pre‐test and a much smaller degree of improvement on the post‐test. Proposes that academic libraries should participate in the First‐Year Experience programs on their campuses. These activities reduce the levels of library anxiety felt by first‐year students and reduce possible barriers to academic achievement. Concludes that this paper will be of use to librarians seeking administrative and campus‐wide support for inclusion of a library component in First‐Year Experience programs. The survey can be used to assess efficacy of activities for those libraries already participating in such programs.

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