The C57BL/6 mouse strain is a justifiable model system for studying the mechanisms involved in ethanol regulation of mesolimbic dopamine activity. Habituation to intraperitoneal injection should be used in male C57BL/6 mice for experiments in which the dopamine response is measured after intraperitoneal injection of a drug. The dissociation between dopamine and ethanol may indicate an acute neural adaptation to ethanol-induced dopamine response in the ventral striatum after a single ethanol injection.
Because the cot-2 and inv loci of Neurospora crassu are closely linked, the invertase from the morphological mutant, cot-2, was examined. The cot-2 strains produce an invertase with altered heat sensitivity, K,, and ratio of heavy to light forms. The cellular localization of cot-2 invertase is different from that of the wild type. There were no observable changes in the energy of activation or the pH optimum of cot-2 invertase, and some of the differences detected were not apparent under culture conditions that promoted wild-type growth. Since recombination (about 5 %) occurred between cot-2 and inv and culture conditions affected the enzyme characteristics, we suggest cot-2 determines, in part, the carbohydrate composition of the enzyme.
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