SUMMARYThe tritium labeled form of 5-amino-7-(2-phenylethyl)-2-(2-furyl)- pyrazolo[4,3-e] clpyrimidine with tritium gas in the presence of 10% Pd-C. Final product was purified by HPLC to give the title 3H-SCH 58261 with radiochemical purity of 99% and specific activity of 68.6 Ci/mmol. 3H-SCH 58261 bound A~A receptors in rat striatal membranes (specific binding > 90%) with Kd and Bmax value of 0.70 nM and 971 fmoYmg of protein, respectively. 3H-SCH 58261 represents a useful tool for a further characterization of A~A adenosine receptor sub type.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.