TOMAŠKIN JÁN, JANČOVIČ JÁN, VOZÁR ĽUBOŠ, TOMAŠKINOVÁ JUDITA: The eff ect of mineral fertilization on belowground plant biomass of grassland ecosystems. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2013, LXI, No. 5, pp. 1431-1440 Aim of the work was to determine the eff ect of diff erent doses of mineral fertilization on belowground and aboveground plant biomass production of three diff erent types of grasslands, to state R:S ratio (root:shoot) and turnover period of belowground plant biomass of grasslands. In the contribution, we assess production of underground biomass, tillering zone and aboveground biomass on three types of grasslands -permanent grassland (PG), over-sown grassland (OSG) and temporary grassland (TG) in sub-mountain area of central Slovakia. There were applied four levels of mineral nutrition in each grassland (non-fertilized variant, var. 30 kg.ha −1 P and 60 kg.ha −1 K. var. 90 kg.ha −1 N + P 30 K 60 , var. 180 kg.ha −1 N + P 30 K 60 ). The root biomass has the most signifi cant share in the total biomass of grasslands (49.9-54.2 %), followed by tillering zone (33.3-36.0 %) and with the lowest share of aboveground biomass (11.9-16.8 %). A dominant share of root biomass and tillering zone ensure signifi cant extraproductive functions of grasslands that contribute to the stability of agriculture landscape. We recorded the lowest amounts of root mass on TG (7.31 t.ha ). The specifi c nitrogen stimulating infl uence on root biomass production has been proven. Production of tillering zone was lower on OSG and TG (5.11 or 5.42 t.ha −1 ), signifi cantly higher on PG (5.72 t.ha −1 ). We observed a signifi cantly higher production of tillering zone with variants which were fertilized with nitrogen than on non-fertilized and PK fertilized. The lowest harvests of aboveground biomass were noticed on TG (5.80 t.ha −1 ), signifi cantly higher on PG and OSG (6.35 or 6.54 t.ha −1 ). Mineral nutrition had a signifi cant impact on production of aboveground biomass. R:S ratio of the assessed grasslands achieved the values from 4.02 to 5.16. Higher values on PG (5.16) are indicating its higher resistance to drought. Turnover time of root biomass was the longest on PG 3.5-5.0 years, on OSG and TG 2.5-3.5 years. Based on achieved results, we recommend using the fodder plants cultivation system on PG or OSG. Permanent grasslands are proved as ecologically more stable and more resistant to drought than temporary grasslands; they can together with optimal mineral nutrition provide adequate production of root biomass (8.5 t.ha −1 ) and a harvest of aboveground biomass (6.3 t.ha −1 ). aboveground biomass, grass sod, grassland, plant biomass, root, R:S ratio, tillering zone, turnover time of root biomass Grass ecosystems are providing besides a productive function also signifi cant extraproductive functions (erosion control, retention, biofi ltration, environmental). Grass sod (root biomass and tillering zone) provides these functions and makes a dominant 80-85 % share in total product...