Factors influencing Agrobacterium tumefaciens-mediated genetic transformation of plants have been widely reported: type and concentration of antibiotic, co-culture period, concentration of bacteria, concentration of acetosyringone, and of course, the type and age of the explants, as well as temperature conditions. However, it is not yet understood how these factors interact and how they affect the efficiency of the final transformation. The aim of this work was to evaluate the interaction of the three main factors affecting the transformation of chrysanthemum (Dendranthema grandiflora) var. Micromargara. A 2 3 factorial design was used with central points, in which three concentrations of A. tumefaciens (1.0, 1.5. and 2.0 of D.O. 600 ), three concentrations of acetosyringone (50 μM, 75 μM and 100 μM), and three co-culture periods (1, 2 and 3 days) were evaluated. The transformation was verified by GUS staining and by means of RT-PCR amplification and sequencing of fragment of the genes uidA, aph3 II and act-cr. The results show that, among the factors evaluated, only the concentration of A. tumefaciens presented a statistically significant effect (p< 0.05) on transformation efficiency, without interaction with the factors of acetosyringone concentration and co-culture period. It is concluded therefore that an A. tumefaciens concentration of D.O 600 : 2.0 is determinant for greater transformation efficiency in chrysanthemum var. Micromargara.
This article contains metadata related to the research article “Behavior of genetic diversity in F1 crosses of selected accessions of Jatropha curcas” (Sánchez-Velázquez et al., 2018). The data presented in this article belong to a diversity study using ISSR molecular markers of a J. curcas germplasm collection that includes bred offspring and analysis of similarity between accessions. We tested previously reported primers in PCR assays to obtain a genetic profile of the accessions. These profiles were used to calculate Dice similarities. Similarity between offspring and parentals can be compared either with the maternal side or paternal side.
<p><strong>Background.</strong> The morphological characterizations and varietal descriptions of <em>Jatropha curcas</em> do not consider all morphological variability of this species. The phenotypes of <em>J. curcas</em> that have been described represent only a part of the variability of this species, making it difficult to distinguish between accessions based on morphological traits. <strong>Objective.</strong> Propose a method to assign trait states to discriminate between accessions based on their phenotype, and to evaluate the efficiency of these phenotypes as morphological traits. <strong>Methodology.</strong> Thirteen morphological traits were selected, and trait states were assigned according to their variability. <strong>Implications.</strong> The results show that some traits, such as the number of male flowers and the ratio of male to female flowers provide redundant information, while other traits do not provide information with enough discriminatory power. By other hand, trait associated with the frequency of one, two, three or four seeds per fruit set the pattern for the identification of phenotypes. <strong>Conclusion.</strong> The proposed method allowed to use the variability of traits as a descriptor without limiting the use of morphological traits not included in this work. </p>
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