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Efforts to improve cacao value chains often assume that production of fine and flavor varieties will raise smallholder incomes. Undertaking postharvest practices of fermentation and drying is another exercise assumed to increase value‐added capture by smallholder producers. This study employs household data from 340 cacao farms in 15 villages in coastal Ecuador during 2018 to empirically assess these assumptions. Contrary to the common belief of the international development community, we find that the production of fine and flavor cacao varieties has no association with the price received by small‐scale producers. This is mainly due to (i) low productivity and (ii) nonexistent price premiums. Findings also suggest that the use of postharvest practices of fermentation and drying may lead to substantial price responses irrespective of the type of variety grown. The results presented here have implications for program interventions aimed at increasing farmer revenue. Programs promoting the use of fine and flavor varieties alone might be misguided and can be improved by training in modern processing techniques, regardless of the variety produced. [EconLit Citations: O12, Q01, Q12, Q13].

Background Microglia participate in the immune response upon central nervous system (CNS) infections. However, the role of these cells during herpes simplex encephalitis (HSE) has not been fully characterized. We sought to identify different microglia/microglia-like cells and describe the potential mechanisms and signaling pathways involved during HSE. Methods The transcriptional response of CD11b+ immune cells, including microglia/microglia-like cells, was investigated using single-cell RNA sequencing (scRNA-seq) on cells isolated from the ventral posterolateral nucleus (VPL)-enriched thalamic regions of C57BL/6 N mice intranasally infected with herpes simplex virus-1 (HSV-1) (6 × 105 PFUs/20 µl). We further performed scanning electronic microscopy (SEM) analysis in VPL regions on day 6 post-infection (p.i.) to provide insight into microglial functions. Results We describe a novel microglia-like transcriptional response associated with a rare cell population (7% of all analyzed cells), named “in transition” microglia/microglia-like cells in HSE. This new microglia-like transcriptional signature, found in the highly infected thalamic regions, was enriched in specific genes (Retnlg, Cxcr2, Il1f9) usually associated with neutrophils. Pathway analysis of this cell-type transcriptome showed increased NLRP3-inflammasome-mediated interleukin IL-1β production, promoting a pro-inflammatory response. These cells' increased expression of viral transcripts suggests that the distinct “in transition” transcriptome corresponds to the intrinsic antiviral immune signaling of HSV-1-infected microglia/microglia-like cells in the thalamus. In accordance with this phenotype, we observed several TMEM119+/IBA-I+ microglia/microglia-like cells immunostained for HSV-1 in highly infected regions. Conclusions A new microglia/microglia-like state may potentially shed light on how microglia could react to HSV-1 infection. Our observations suggest that infected microglia/microglia-like cells contribute to an exacerbated CNS inflammation. Further characterization of this transitory state of the microglia/microglia-like cell transcriptome may allow the development of novel immunomodulatory approaches to improve HSE outcomes by regulating the microglial immune response.

We apply a value chain approach to examine how blackberry producers in Ecuador can improve production and marketing practices to obtain more favorable prices. Results show that general upgrading actions are associated with improved producer prices, and specific activities, such as seeking information from reputable sources can lead to substantial welfare improvements. The paper goes beyond most value chain studies which treat participation in a 'modern' value chain as a discrete state. Ecuador's blackberry industry is evolving incrementally with no clearly discernible 'modern' market chains. Instead, producers incrementally make improvements in multiple dimensions. Relatively low-cost practices, many of which do not imply substantial risk or investment, are associated with price improvements. Obstacles to participation in incremental value chain upgrading activities are not insurmountable. In particular, scale of production is not a limiting factor: small-scale producers are not precluded from adopting improved practices and they do not suffer in terms of price received.

Naranjilla cultivation is highly profitable in many parts of the Andean foothills in Colombia and Ecuador. Its susceptibility to soil-borne diseases, however, lowers its economic benefits, reduces sustainability of production and increases its contribution to environmental degradation. This paper presents an analysis of the potential market and non-market benefits of research that developed and tested the grafting of common naranjilla onto disease-resistant rootstock. Grafting reduces the need for pesticide application and increases the longevity and sustainability of the plant. An economic surplus approach, carefully calibrated to reflect the realities of naranjilla production, was employed to show the large benefits from such research. Environmental and health benefits are very close in magnitude to market-mediated surplus gains. The results show substantial potential benefits from an outreach program to diffuse the new technology.