The DNA barcode reference library for Lepidoptera holds much promise as a tool for taxonomic research and for providing the reliable identifications needed for conservation assessment programs. We gathered sequences for the barcode region of the mitochondrial cytochrome c oxidase subunit I gene from 160 of the 176 nominal species of Erebidae moths (Insecta: Lepidoptera) known from the Iberian Peninsula. These results arise from a research project which constructing a DNA barcode library for the insect species of Spain. New records for 271 specimens (122 species) are coupled with preexisting data for 38 species from the Iberian fauna. Mean interspecific distance was 12.1%, while the mean nearest neighbour divergence was 6.4%. All 160 species possessed diagnostic barcode sequences, but one pair of congeneric taxa (Eublemma
rosea and Eublemma
rietzi) were assigned to the same BIN. As well, intraspecific sequence divergences higher than 1.5% were detected in four species which likely represent species complexes. This study reinforces the effectiveness of DNA barcoding as a tool for monitoring biodiversity in particular geographical areas and the strong correspondence between sequence clusters delineated by BINs and species recognized through detailed taxonomic analysis.
The purpose of this study was to evaluate the safety, efficacy, and efficiency of a Descemet membrane endothelial keratoplasty (DMEK) graft preparation device, DescePrep, through measurement of graft viability, yield, and preparation time in both healthy and diabetic (high-risk) donor eyes.Methods: Twenty nondiabetic and 10 diabetic donor corneas were processed using DescePrep, which standardizes the liquid bubble technique. Corneas were stained with trypan blue and then processed. Cell counts through specular microscopy, optical coherence tomography imaging, and slit-lamp analysis were used for the evaluation of graft separation and viability in 5 nondiabetic corneas. The remaining 25 corneas (15 nondiabetic and 10 diabetic) were evaluated for preparation success rate and processing time. Ten corneas (5 nondiabetic and 5 diabetic) were randomly selected for further evaluation of global cell loss through staining.Results: Ninety-seven percent of corneas (29 of 30) were prepared successfully with DescePrep. The average preparation time was 2.83 6 1.8 minutes. There was no significant difference in the time of preparation between the nondiabetic and diabetic groups (P = 0.077). The overall average cell death after processing was 7.9% 6 3.7% for all corneas. There was no significant difference in cell viability between diabetic and nondiabetic tissues after Desce-Prep processing (P = 0.769).
Conclusions: DescePrep is a new DMEK preparation techniquethat can process both nondiabetic and diabetic donor corneas at high yields in minutes. High-yield preparation of diabetic corneas may offer eye banks access to a larger donor pool, which is important because the demand for DMEK grafts continues to rise worldwide.
Se han identificado, hasta el momento, 70 especies pertenecientes a las familias Cossidae, Limacodidae, Lasiocampidae, Saturniidae, Sphingidae, Drepanidae, Notodontidae, Nolidae y Erebidae en el Parque Natural Sierra María-Los Vélez (Almería, España). Los elementos de distribución mediterránea son mayoritarios (65,7%) e incluyen 3 endemismos ibéricos. El corotipo euroasiático está relativamente bien representado con un 17,1%. Las estrategias fenológicas de las especies son principalmente bivoltina (48,6%) y univoltina (45,7%) frente a la polivoltina del resto de especies (5,7%). Los taxones que se citan por primera vez en la provincia de Almería son 20 especies.
The Murcia Region (south-eastern Iberian Peninsula) has a great diversity of Lepidopteran fauna, as a zoogeographical crossroads and biodiversity hotspot with more than 850 butterflies and moth species recorded.
In the present paper, based on an examination of museum specimens, published records and new samples, a comprehensive and critical species list of Pyralidae moths (Lepidoptera, Pyraloidea) is synthesised. In total, three subfamilies, 67 genera and 142 species have been recorded and these are listed, along with their collection, literature references and biological data, including chorotype, voltinism and the flight period in the study area. The subfamilies are Galleriinae, Phycitinae and Pyralinae. Seventy-three species are newly recorded, sixty-two species are confirmed from literature and only seven species have not been observed for the Murcia Region.
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