Anatomical and physiological specializations for plant adaptation to harsh climates are the results of molecular mechanisms that can be nuclear or organellar encoded. In this study, the complete plastomes of an arctic species, Oxytropis arctobia Bunge (Fabaceae,) and a closely related temperate species, O. splendens Douglas ex Hook., were assembled, annotated and analyzed to search for differences that might help explain their adaptation to different environments. Consistently with the previously sequenced O. bicolor DC. and O. glabra plastomes, the O. arctobia and O. splendens plastomes both have the common features of the inverted repeat-lacking clade (IRLC), as well as the atpF intron loss, which is unique to the genus. However, significant differences distinguishes the O. arctobia from O. splendens and other closely related plastomes (Oxytropis spp. and Astragalus spp.), including a 3 kb inversion, two large insertions (>1 kb), significant modifications of the accD gene, and an overall larger size.