The aim of this research was to determine the influence of temperature, agitation, sludge concentration, and solids retention time (SRT) to obtain readily biodegradable organic matter on primary sludge (PS) fermentation, which would be used as substrate in a biological nutrient removal (BNR) process. Stirring and heating the sludge as well as increasing SRT improved the PS fermentation, producing a large amount of soluble chemical oxygen demand (SCOD). The influence of each operational parameter on PS hydrolysis was observed clearly. A great performance on SCOD production was obtained when the PS was stirred and heated for 3 days. However, PS concentration did not affect the fermentation. Sludge agitation is a simple process with minimal energy consumption. Warming the sludge is very interesting in those plants with anaerobic digestion, where heat energy is obtained from biogas. Therefore, PS fermentation can be improved with a minimum investment and leveraging existing resources in a wastewater treatment plant (WWTP). Fermenter volume can also be reduced if sludge is being heated and stirred during fermentation.
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