Anaerobic digestion process is gaining attention in the agriculture industry because of its potential for renewable energy production and manure stabilization. These potential benefits are significant against the current backdrop of rising energy costs and growing environmental concerns. Anaerobic digestion is a naturally occurring process through which organic matter such as manure, feed spills, meat processing wastes and crop residues are stabilized by microorganisms strictly in the absence of air. In developing countries, the inadequate management of waste particularly in urban areas where the consumption pattern have changed and the generation rate increased substantially from one location to another and from time to time. This study explored the production of biogas from kitchen wastes mixed with intestinal cow dung in a semi continuous anaerobic digester. Materials used as feed were Paw Paw, tomato, banana peels and intestinal cow dung. Various parameters were determined using standard methods; water displacement method was used to determine the volume of gas produced. The combustibility of the gas generated was tested using Bunsen burner. Microbiological methods were employed for the microbiological analysis. The temperature and pH recorded in the digester range from 27-33oC and 0-6.81c respectively. It was observed that the waste slurry produced biogas without nutrient or chemical addition to the digester. The highest volume of gas (2.8ml) was produced on the 14th day where the temperature (29oC) and pH (6.79) were relatively high. The result of the statistical analysis showed there is significant difference (P<0.05) in the slurry temperature and pH as compared to the volume of gas produced. The Bacteria isolates identified from the digester include Proteus Spp, Bacillus Spp, Escherichia Coli, Klebsiella Spp and Staphyllococcus Spp. Based on this, the search for alternative source of Energy such as Biogas should be intensified so as to curb the incidence of ecological disasters like Environmental pollution, deforestation, desertification and erosion.
The Timber Market is a place where timber is processed and sold, there is also a provision for welding work and sales of timber allied products, offloading of woods, restaurant, etc.The woods are supplied from various states across the country including Edo, Cross River, Delta, Ondo, Akwa Ibom states of Nigeria by land transportation, thus the use of trailers, lorries etc being a necessity.The woods are processed and prepared for sale using different machines like the table saw, planning machines, mortising and grooving machine, filing machines and the most important and main machine which is the spindle(sterner) machine.
Dehydrogenase and inhibitions of dehydrogenase activities in Salmonella typhimurium by ethanolic and methanolic leaf extracts of Ocimum gratissimum and Carica papaya were investigated. Dehydrogenase activity assay was carried out using 2, 3, 5-triphenyl tetrazolium chloride (TTC) as the electron acceptor. Pure culture of S. typhimurium was exposed to varied concentrations of ethanolic and methanolic extracts of Ocimum gratissimum and Carica papaya [0-4000 μg/ml]. The ethanolic and methanolic extracts exhibited a concentration dependent response against the tested organism. Results obtained revealed that the ethanolic extracts showed a higher bactericidal effect on the test organism than the methanolic extracts at the threshold and total inhibitory concentrations. The IC50 were 45.349 and 15.697; IC100 were 43.732 and 35.526 for ethanolic extracts of Carica papaya and Ocimum gratissimum respectively while the IC50 were 7.108 and 13.696; IC100 were 40.815 and 31.104 for methanolic extracts of Carica papaya and Ocimum gratissimum respectively. This in-vitro study further revealed that the leaf of Carica papaya was more potent on Salmonella typhimurium than the leaf of Ocimum gratissimum. The findings from this study seem to provide the in-vitro evidence that justifies Carica papaya and Ocimum gratissimum as good candidate medicinal plants for the potential treatment of Salmonella typhimurium infections.
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