Early neutrophil entry into an inflammatory site is thought to mediate a chemokine switch, inducing subsequent monocyte recruitment through the regulation of monocyte chemoattractant protein-1 (MCP-1) release. As the murine monocyte is poorly characterized and difficult to identify, there has been little examination of either its early recruitment in inflammatory models or of the factors that influence its early migration. The phenotyping of rapidly recruited inflammatory leukocytes with 7/4 and Gr-1 monoclonal antibodies (mAbs) identifies 2 distinct populations, which we characterize as murine monocytes and neutrophils. Monocytes migrate in the first 2 hours of inflammation making use of ␣41 but not of Mac-1 or lymphocyte function-associated antigen-1 (LFA-1)
relative to Freelite (Figure 1). Both patients had negative results for urine BJPs, further undermining the validity of the ELISA assay results. It is possible that the monoclonal antibodies are cross-reacting with the reported IgG λ and IgA λ monoclonal intact immunoglobulins in these patients, leading to falsely raised λ FLC results. Suggestions by Davern and colleagues 1 that Freelite antisera regularly underestimate results due to missing a wide variety of λ subgroups, are affected by instrumentation and "unknown" technical issues, and are confounded by interference from intact immunoglobulins are not supported by their data. The results reported and views expressed in the article should be compared with the scientific consensus in the many, larger, and more rigorous evaluations of Freelite already published.
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