The bacteria of the genus Staphylococcus present as Gram-positive cocci, grouped in clusters and are mainly found in normal human flora (skin and mucosa). They are classified by the ability to clot plasma coagulase positive or negative coagulase. The coagulase-negative Staphylococci (CNS) have become relevant in the clinic after the identification of these microorganisms in infections. The emergence of antimicrobialresistant strains in hospitals raises concerns regarding these microorganisms. This study aimed to evaluate the frequency of nasal colonization by CNS and its resistance profile methicillin professionals of a health institution in the region northern of the Espirito Santo. Samples were collected from the nasopharynx of volunteers, isolated Mannitol Salt Agar and identified by Gram staining technique, the catalase test and free coagulase. The methicillin resistance profile was evaluated by susceptibility of the organism against the oxacillin disk. Of the 38 samples collected, isolated in 31 (81.6%) the CNS. Of 30 samples CNS assayed for sensitivity to oxacillin, 23 (76.7%) showed resistance. It was found that professionals in direct contact with patients have more frequently resistant CNS. Therefore, it is considered important to the care of the spread of resistant strains of microorganisms by professionals in a hospital setting, avoiding causing major damage to the health of patients and higher expenses for health services.