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Prolonged high-fat diets (HFDs) can cause intramyocellular lipid (IMCL) accumulation that may negatively affect muscle function. We investigated the duration of a HFD required to instigate these changes, and whether the effects are muscle specific and aggravated in older age. Muscle morphology was determined in the soleus, extensor digitorum longus (EDL) and diaphragm muscles of female CD-1 mice from 5 groups: young fed a HFD for 8 weeks (YS-HFD, n=16), young fed a HFD for 16 weeks (YL-HFD, n=28) and young control (Y-Con, n=28). The young animals were 20 weeks old at the end of the experiment. Old (70 weeks) female CD-1 mice received either a normal diet (O-Con, n=30) or a HFD for 9 weeks (OS-HFD, n=30). Body mass, body mass index and intramyocellular lipid (IMCL) content increased in OS-HFD (P≤0.003). In the young mice, this increase was seen in YL-HFD and not YS-HFD (P≤0.006). The soleus and diaphragm fibre cross-sectional area (FCSA) in YL-HFD was larger than that in Y-Con (P≤0.004) while OS-HFD had a larger soleus FCSA compared with that of O-Con after only 9 weeks on a HFD (P<0.001). The FCSA of the EDL muscle did not differ significantly between groups. The oxidative capacity of fibres increased in young mice only, irrespective of HFD duration (P<0.001). High-fat diet-induced morphological changes occurred earlier in the old animals than in the young, and adaptations to HFD were muscle specific, with the EDL being least responsive.

This study examined the effects of the Badminton World Federation (BWF) Shuttle Time program on fundamental movement skills (FMS) in English children. A total of 124 children; 66 in key stage 1 (ages 6–7 years) and 58 in key stage 2 (10–11 years) undertook the Shuttle Time program, once weekly for six weeks (n = 63) or acted as controls (n = 61). Pre, post and ten-weeks post, both process and product FMS were determined. Children in the intervention group, aged 6–7 years, had higher total process FMS (via test of gross motor development-2) compared to the control group at post and ten-weeks post intervention (both p = 0.0001, d = 0.6 and 0.7, respectively). There were no significant differences in process FMS scores for children aged 10–11 years. Ten-meter sprint speed decreased pre to post and was maintained at ten-weeks post for the intervention groups aged 6–7 years (p = 0.0001, d = 0.6) and 10–11 years (p = 0.001, d = 0.2) compared to control. Standing long jump distance increased pre to post (p = 0.0001, d = 0.8) and was maintained at ten-weeks post (p = 0.0001, d = 0.5) for the intervention group. Medicine ball throw performance increased pre to post (p = 0.0001, d = 0.3) for the intervention group. The BWF Shuttle Time program is beneficial in developing FMS for key stage 1 children (ages 6–7).

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In exploring the mechanism of action of vasodilating drugs an attempt has been made to study the enzyme systems of the blood vessels. A transphosphatase of the myokinase type has been found to be present in the coronary arteries of the steer. The activity has been studied under the optimal conditions for this enzyme system. Coronary arterial tissue may be maintained at the temperature of dry ice for a number of days without significant loss of enzyme activity. This will permit an investigation of the effects of drugs upon the activity of these enzymes.

The current study sought to examine the construct validity of the Resistance Training Skills Battery for Children (RTSBc), a movement screen purported to assess resistance training skill in children. Children aged 7-10 years (n = 27, 21 males, 6 females) undertook measures of resistance training skill via the RTSBc, motor competence and muscular fitness. Using a median split for RTSBc scores, children were categorised as high or low resistance training competence. Univariate ANCOVAs, controlling for maturation, were used to examine whether measures of muscular fitness and motor competence scores differed as a function of RTSBc competence. Children who were classified as high for resistance training competence had significantly better motor competence (P = .001) and significantly faster 10 m sprint speed (P = .001). However, medicine ball throw and standing long jump scores as well as peak and average isokinetic muscle strength did not differ as a function of RTSBc (P > 0.05). In all cases maturation was significant as a covariate. This study is the first to demonstrate construct validity of the RTSBc as a measure of general motor competence and sprint speed, but not strength, in children aged 7-10 years.

The present study examined if 20-weeks high-fat diet (HFD) consumption had a temperature specific effect on the contractile performance and regional thermal sensitivity of isolated mouse soleus (SOL) and diaphragm (DIA) muscle. Four-week-old female CD-1 mice were randomly selected to consume either a standard laboratory diet or a standard laboratory diet in conjunction with a HFD for 20-weeks. Peripheral SOL and core DIA were isolated from each animal and maximal isometric force and work loop power were assessed at 20⁰C, 28⁰C, 35⁰C and 40⁰C. Increasing temperature to 35⁰C resulted in greater isometric stress, lower activation and relaxation time and higher work loop power in both muscles. A further increase in temperature to 40⁰C did not affect isometric force but increased work loop power output of the SOL. Conversely, isometric force of the DIA was reduced and work loop power maintained when temperature was increased to 40⁰C. HFD consumption resulted in greater isometric force and absolute work loop power of the SOL and reduced isometric stress of the DIA, effects that were less apparent at lower temperatures. When the relationship between temperature and each measure of contractile function was examined by linear regression, there was no difference in slope between the control or HFD groups for either SOL or DIA. These results indicate that whilst contractile function initially increases with temperature, the temperature to elicit maximal performance is muscle and contractile mode-specific. Furthermore, HFD effects on contractile function are temperature specific, but HFD does not influence the relationship between temperature and performance.

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