F Acid‐Containing Phospholipids in Sugar Cane Cells
Heterotrophic suspension cultures of sugar cane (Saccharum spec.) cells contain the F acids 1b–f. These acids are bound preferably to phosphatidylethanolamine 3 and phosphatidylcholine 4. Structures of F acid‐containing phospholipid molecular species 3b–d, 4b and 4c were established by enzymatic positional hydrolysis and GC/MS analysis of the respective (trimethylsilyl)diacylglycerol derivatives 5.
Origin of the Methyl Substituents of F Acids
Feeding experiments with L‐[methyl‐13C]methionine on suspension cultures of Saccharum spec. demonstrate that the furan methyl groups of F acids 1 are derived from methionine. Specific incorporation of 13C label was confirmed by GC/MS analysis of the tetrahydrofuran derivatives 2.
Origin of the Basic Carbon Chain of F Acids
Feeding experiments with sodium [2‐13C]acetate on suspension cultures of Saccharum spec. show that the basic carbon chain of F acids 1 is derived from acetate units via the fatty acid pathway. Incorporation of 13C label was confirmed by GC/MS analysis of the corresponding tetrahydrofuran derivatives 2.
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