Brown adipocytes differentiated in primary cell culture were found to contain a type II iodothyronine 5'‐deiodinase (5'D), Incubation of confluent cells with norepinephrine or dibutyryl‐cAMP caused up to 17‐fold increase in 5'D activity with a maximum after 8 h. Activation of 5'D required mRNA and protein synthesis and was accompanied by parallel, up to 5.8‐fold increase in the amount of mitochondrial uncoupling protein with a maximum after 24 h. Analysis ofadrenergic stimulation of 5'D suggested predominant involvement of the β‐receptors and increased intracellular cAMP levels, while the contribution of x1,‐receptors was small.
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