The antimicrobial peptide dermaseptin 01 (DS 01), from the skin secretion of Phyllomedusa hypochondrialis frogs, was immobilized in nanostructured layered films in conjunction with nickel tetrasulfonated phthalocyanines (NiTsPc), widely used in electronic devices, using layer-by-layer technique. The films were used as a biosensor to detect the presence of dopamine (DA), a neurotransmitter associated with diseases such as Alzheimer's and Parkinson's, with detection limits in the order of 10−6 mol L−1. The use of DS 01 in LbL film generated selectivity in the detection of DA despite the presence of ascorbic acid found in biological fluids. This work is the first to report that the antimicrobial peptide and NiTsPc LbL film exhibits electroanalytical activity to DA oxidation. The selectivity in the detection of DA is a fundamental aspect for the development of electrochemical sensors with potential applications in the biomedical and pharmaceutical industries.

The Cerrado is one of the most threatened biomes in Brazil. It is estimated that much of its original vegetation has been replaced by some type of human use. This is quite worrying, mainly in the northern part where the number of wildlife inventories is insufficient and creates the false impression of low diversity in the region. The Parque Estadual do Mirador-PEM, located in the south-central region of the state of Maranhão, presents vegetation typical of the Cerrado biome and corresponds to one of 46 priority areas for conservation in the state. Herein, we describe the species richness and composition of the anurofauna from the PEM and analyze the influence of different types of vegetation in its formation. Our inventory was conducted from December 2013 to February 2015, using the active search and auditory census methods on breeding sites in different water bodies of the park. We recorded 31 anuran species belonging to five families (species number in parentheses): Leptodactylidae (14), Hylidae (12), Bufonidae (3), Microhylidae (1) and Phyllomedusidae (1). The rarefaction curve and species richness estimators indicated that the sampling effort was enough to record most of the species in the region. The richness of anurans in the PEM was higher than reported by other authors for several areas of Cerrado. Most species have a wide distribution in Brazil or are strongly associated with the Caatinga or Amazon biomes. Only about 19% are endemic to the Cerrado biome. This study is the first to inventory the anurans species of the south-central region of state of Maranhão and provides important data on amphibian communities from the northern part of the Brazilian Cerrado.Keywords: inventory, Amphibia, Anura, conservation unit, species richness. Anfíbios anuros do Parque Estadual do Mirador, um remanescente de Cerrado no estado do Maranhão, nordeste do BrasilResumo: O Cerrado é um dos biomas mais ameaçados do Brasil. Estima-se que grande parte da cobertura vegetal original deste bioma tenha sido substituída por alguma forma de utilização humana. Este fato é bastante preocupante, principalmente na porção norte, onde o número de inventários faunísticos é insuficiente e gera a falsa impressão de baixa diversidade na região. O Parque Estadual do Mirador-PEM, localizado na região centro-meridional do Maranhão, apresenta vegetação típica do Cerrado e corresponde a uma das 46 áreas prioritárias para conservação no estado. Aqui, nós descrevemos a riqueza e a composição da anurofauna no PEM, analisando a influência dos diferentes tipos vegetacionais na sua formação. Inventariamos a anurofauna de dezembro de 2013 a fevereiro de 2015, utilizando métodos de procura ativa e censo auditivo em sítios reprodutivos de diferentes corpos d'água do parque. Registramos 31 espécies de anuros, pertencentes a cinco famílias (números de espécies entre parênteses): Leptodactylidae (14), Hylidae (12), Bufonidae (3), Microhylidae (1)

For the prospective immunorecognition of 5-enolpyruvylshikimate-3-phosphate synthase (CP4-EPSPS) as a biomarker protein expressed by transgenic soybean, an extensive in silico evaluation of the referred protein was performed. The main objective of this study was the selection of a set of peptides that could function as potential immunogens for the production of novel antibodies against CP4-EPSPS protein. For this purpose, the protein was in silico cleaved with trypsin/chymotrypsin and the resultant peptides were extensively analyzed for further selection of the best candidates for antibody production. The analysis enabled the successful proposal of four peptides with potential immunogenicity for their future use as screening biomarkers of genetically modified organisms. To our knowledge, this is the first attempt to select and define potential linear epitopes for the immunization of animals and, subsequently, to generate adequate antibodies for CP4-EPSPS recognition. The present work will be followed by the synthesis of the candidate peptides to be incubated in animals for antibody generation and potential applicability for the development of an immunosensor for CP4-EPSPS detection.

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