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. 2 Biólogo, Ms., Doutorando em Entomologia (UFV). 3 Engenheiro Florestal, Ms., Pesquisador (UFV). 4 Engenheiro Agrônomo, Ms., Doutorando em Entomologia (UFV). 7, 15, 30, 60, 90, 120, 150, 180 control, no bait was applied. After 24 and 48 hours the transport and refusal of the baits were evaluated, and after 7, 15, 30, 60, 90, 120, 150, 180 and 210 RESUMO Embora muitos produtos tenham sido testados como princípio ativo de iscas formicidas

A reprodução de parasitóides apresenta etapas interligadas e a idade de hospedeiros e parasitóides pode afetar o desenvolvimento desses últimos. Características biológicas de fêmeas de Trichospilus diatraeae Cherian & Margabandhu (Hymenoptera: Eulophidae), com até 96 horas de idade, foram estudadas em pupas do hospedeiro alternativo Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae). Cada pupa de A. gemmatalis com 0-24, 24-48, 48-72 ou 72-96 horas de idade, foi oferecida ao parasitismo por 10 fêmeas de T. diatraeae com 0-24, 24-48, 48-72 ou 72-96 horas de idade, por 24 horas à 25 ± 2oC, umidade relativa de 70 ± 10% e fotofase de 14 horas. A emergência, o número de descendentes, a duração do ciclo, a razão sexual, a longevidade e largura da cápsula cefálica de machos e de fêmeas de T. diatraeae apresentaram resposta semelhante para o efeito idade das pupas desse hospedeiro e fêmeas desse parasitóide em conjunto. No entanto, houve redução da emergência, número de descendentes e longevidade das fêmeas de T. diatraeae com duração do ciclo se ajustando a uma função quadrática quando se avaliou apenas o efeito da idade do parasitóide. Pupas de A. gemmatalis, com até 96 horas de idade, podem ser usadas para criação massal de T. diatraeae utilizando-se fêmeas desse parasitóide com até 96 horas de idade.Reproduction of Trichospilus diatraeae Cherian & Margabandhu (Hymenoptera: Eulophidae) on Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) Pupae in Relation to  Age of the Parasitoid and HostAbstract. Reproduction of parasitoids presents interconnected steps and the ages of hosts and parasitoids can affect the development of parasitoids. Biological parameters of Trichospilus diatraeae Cherian & Margabandhu (Hymenoptera: Eulophidae), at up to 96 hours of age were studied on pupae of the alternative host Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) pupae. Each A. gemmatalis pupae from 0-24, 24-48, 48-72 or 72-96 hours of age, was offered to parasitism to 10 females of T. diatraeae at 0-24, 24-48, 48-72 or 72-96 hours of age for 24 hours at 25 ± 2°C, relative humidity of 70 ± 10% and photophase of 14 hours. Emergency, number of offspring, length of cycle, sex ratio, longevity and width of cephalic capsule of males and females of T. diatraeae showed similar response when effect of age of pupae and females of parasitoid were evaluate jointly. However, emergency, number of offspring and longevity of females of T. diatraeae were reduced as length of cycle was being adjusted to a quadratic function when only effect of age of parasitoid was evaluated. Pupae of A. gemmatalis with up to 96 hours of age can be used for mass rearing of T. diatraeae using females at up to 96 hours of age.

Diaphania hyalinata L. (Lepidoptera: Crambidae) is a key pest of plants in the family Cucurbitaceae, defoliating and reducing photosynthetic area and yield. Trichospilus diatraeae Margabandhu & Cherian, 1942 (Hymenoptera: Eulophidae) parasitizes the pupae of this pest, but its biological and reproductive parameters with this host are unknown. The objective of our study was to characterize the reproduction of T. diatraeae in D. hyalinata pupae with different densities of this parasitoid. Individual D. hyalinata pupae (48 h old) were exposed to different densities of 7'. diatraeae (1:1, 4:1, 8:1, 12:1, 16:1, 20:1, and 24:1). The parasitoid density did not affect the parasitism and emergence rate of this natural enemy. The progeny, sex ratio, longevity, and head capsule width of T. diatraeae males and females decreased with increasing density, and its life cycle was proportional to density increase of T. diatraeae. Densities of up to eight T. diatraeae individuals per D. hyalinata pupae are favorable for the development of this parasitoid.

Methodology for biological studies of Mantodea in laboratory conditionsThe methodology developed was: a wood box with 92 x 30 x 12 cm; three viais of 3000 ml were connected at the inferior part of the box, where Drosophiki me/000gaster (Diptera: Drosophilidae) flies were put; twenty four viais of 170 ml were put in the upper part of the box and one Mantodea ninph was raised to adult in each vial. This methodology prove to be successfui in raising Acontiotespis perspicua (Mantodea: Mantidae) ; 55% of ninph viabiiity were achived. KEYWORDS: methodology; Mantodea; biological studies. RESUMOA metodologia de criação proposta para a espécie testada, Acontiotespis perspicua (Mantodea: Mantidae) , possibilitou uma viabilidade média de 55% para o estádio ninfal, e pode-se obser var os parâmetros biológicos individualmente.Comprovou-se também que, para a criação de drosófilas, o uso de vidros de 3000 ml com bananas em apodrecimento é eficiente.PALAVRAS-CHAVE: metodologia; Mantodea; biologia.Recebido em 22/02/91 1 Pesquisa financiada pelo CNPq, FINEP/CNPq/BIOAGRO/UFV e FAPEMIO.2 Departamento de Biologia Animal, UFV 36570-000 Viçosa MG. Bolsista do CNPq.