Biodynamic agriculture, which considered biodynamic preparations (BP) and compost as essential to farms sustainability, surged as an alternative almost a century ago. Composting is a way to obtain either biofertilizers or soil amendments, whereas the static piles method reduces energy and cost because no turnings are needed. The present study aims to evaluate the BP effect on physical, chemical and biological properties of static piles compost from prickly pear cactus and moringa crop wastes (regional principal substrates) over 100 days of composting. The experiment was carried out in an organic farm (Nuevo León, Mexico) considering four treatments: T1, Prickly pear cactus+BP; T2, Moringa+BP; T3, Prickly pear cactus and T4, Moringa. Results showed significantly higher bacterial activity (p<0.05) in T1 (until 1.38x1010 CFU), therefore it had the highest temperatures and mineralization. Treatments with prickly pear cactus attained the highest temperatures, compared with those with moringa (significantly in 71% of total sampling days, p<0.05). An aerobic environment was maintained by the passive aeration system (holed PVC pipes placed at the bottom layer). The final material was considered to be sanitized, according to Enterobacteriaceae, Escherichia coli and Salmonella/Shigella analysis for quality control. Results indicate the BP efficiency on regional substrates decomposition, by using the static piles method.
Toxoplasma gondii is a protozoan parasite with a broad ecological valence, which has been detected in a wide range of hosts and landscapes. Although the genus is considered monospecific, in recent years it has been demonstrated to exhibit more genetic variability than previously known. In Mexico, there are few genotyping studies, which suggest that classical, autochthonous, and atypical strains are circulating. The goal of this study was to describe T. gondii genetic diversity in naturally infected sheep from Colima, Mexico. This is a good site to study ecological aspects of this parasite since it is located between the Nearctic and Neotropical ecozones and it includes domestic and wild risks for transmission. We analyzed 305 tissue samples of semicaptive sheep from six coastal and central zones of Colima and border zones of Michoacán. We used an 803 bp amplicon of the B1 gene to genotype T. gondii and seroprevalence was determined by ELISA. Indexes for genetic diversity and genetic differentiation were calculated and compared with reference strains from North America (NA) and South America (SA). Twenty-three tissue samples were positive for the B1 gene by PCR, which were sequenced. Crude prevalence was 24.4%. The genetic analysis showed 16 variable sites along the 803 bp region that grouped all sequences into 13 haplotypes in the phylogenetic tree. Bayesian and haplotype network analysis showed nine new B1-types, of which three were frequent and six had unique alleles. Comparisons among sequence sets revealed that the Mexican population had lower differentiation than SA and an intermediate genetic variability between South America and North America. The B1 gene analysis showed new T. gondii haplotypes in naturally infected sheep; therefore, this marker could be initially used in molecular screening studies to identify potentially virulent genotypes of this parasite using natural host samples directly.
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