Molecular data generation and their combination in penile carcinomas (PeCa), a significant public health problem in poor and underdeveloped countries, remain virtually unexplored. An integrativemethodology combin ing genome-wide copy number alteration, DNA methylation, miRNA and mRNA expression analysis was performed in a set of 20 usual PeCa. The well-ranked 16 driver candidates harboring genomic alterations and regulated by a set of miRNAs, including hsa-miR-31, hsa-miR-34a and hsa-miR-130b, were significantly associated with over-represented pathways in cancer, such as immune-inflammatory system, apoptosis and cell cycle. Modules of co-expressed genes generated from expression matrix were associated with driver candidates and classified according to the over-representation of passengers, thus suggesting an alteration of the pathway dynamics during the carcinogenesis. This association resulted in 10 top driver candidates (AR, BIRC5, DNMT3B, ERBB4, FGFR1, PML, PPARG, RB1, TNFSF10 and STAT1) selected and confirmed as altered in an independent set of 33 PeCa samples. In addition to the potential driver genes herein described, shorter overall survival was associated with BIRC5 and DNMT3B overexpression (log-rank test, P = 0.026 and P = 0.002, respectively) highlighting its potential as novel prognostic marker for penile cancer.
CONTEXT: Posterior urethral valve (PUV) is a widely known condition affecting males that generally presents prenatally or at birth. PUVs have also been occasionally described in literature in cases diagnosed during adolescence or adulthood. CASE REPORT: This report presents two late PUV cases, one in a teenager and the other in an adult. Both cases had had clinical signs of urinary tract infection and obstructive urinary symptoms. The diagnoses were made by means of voiding cystourethrography and urethrocystoscopy. Endoscopic valve fulguration was the treatment chosen for both. Their follow-up was uneventful.
INTRODUCTION:
Ionizing radiation-producing equipment is used in surgical centers to guide invasive procedures. Technological advances have enabled improvements in image quality, which may be accompanied by increased radiation doses in the surgical team. Correct use of personal protective equipment and monitoring of radiation levels are required to a safe practice.
OBJECTIVES:
To evaluate radiation exposure conditions in occupationally exposed persons working at the Surgical Center at Hospital das Clínicas da Faculdade de Medicina de Botucatu for implementation of radiation protection measures.
METHODS:
Three different types of fluoroscopy equipment were used: C-arms, a dosimetric system with ionization chambers, and optically stimulated dosimeters. A three-stage evaluation was conducted, consisting of a first stage for observation, a second stage for estimation of kerma rate simulating exposure conditions, and a final stage for dosimetry to estimate the effective dose in workers.
RESULTS:
The most frequent procedures and the disposition for each team member were determined. Kerma values were estimated for both the principal physician and the assistant physician. The maximum number of annual procedures was also estimated so that the dose limits are not exceeded.
CONCLUSIONS:
Dosimetry for the surgical team is indicated as an approach to monitor occupational dose levels. The dose rates and effective dose found in this study are low but not negligible. Thus, proper use of equipment and periodic training for workers are still the best options for radiation protection.
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