Endoglucanase and exocellobiohydrolase produced b Lentinula edodes (Berk.) Pegl. strain thatt was cultivated in carboxymetilcellulose (CMC) or microcrystalline cellulose (Avicel) liquid media. The concentration and type of cellulose influenced the enzyme activity and production. Extra-cellular cellobiase activity was not detected in CMC or Avicel media. This enzyme was detected in mycelial extracts only. With 1.7% Avicel liquid medium, the strain did not produce exocellobiohydrolase, but 74 µmol RBBR/mg protein/min was detected with 0.5% Avicel. The substitution of Avicel by 0.5% CMC reduced this activity. Endoglucanase also had maximum activity in 0.5% Avicel medium (approximately 820 UI/mg protein) after 96 h incubation. In supernatants from 0.5% CMC, the maximum activity attained was 200 UI/mg protein only.
Neste trabalho foram estudadas as atividades de endoglucanase, exocelobiohidrolase e celobiase em uma linhagem de Lentinula edodes (Berk.) Pegl. cultivada em meio líquido contendo carboximetilcelulose (CMC) ou celulose microcristalina (Avicel). Foram detectadas as atividades de endoglucanase e exocelobiohidrolase no sobrenadante das culturas crescidas tanto em meios contendo CMC como nos meios contendo Avicel, sendo observada a influência da concentração e do tipo de celulose. Não foi detectada atividade de celobiase nos sobrenadantes, sendo a mesma detectada somente no extrato micelial. Com uma concentração de 1,7% de Avicel, a linhagem estudada não demonstrou atividade de exocelobiohidrolase. Porém, à concentração de 0,5% obteve-se uma atividade de 74 µmol de RBBR/mg de proteína/min. Com a substituição de Avicel por CMC a 0,5%, a atividade de exocelobiohidrolase foi reduzida a menos de 50%. A máxima atividade de endoglucanase em sobrenadantes obtidos em meio com Avicel a 0,5% foi em torno de 800 UI/mg de proteína, após 96 horas de cultivo. Em sobrenadantes obtidos de meio com CMC, a atividade desta enzima foi de apenas 200 UI/mg de proteína
Studies on the effectiveness of species of the genus Melaleuca (Myrtaceae) against mastitis-causing bacteria, particularly Staphylococcus aureus, have focused on the chemical components of the essential oil. The goal of this work was to use GC-MS analysis to characterize the phytochemical composition of the hexane extract from Melaleuca leucadendra (L) L. leaves. There was evidence of 37 secondary metabolites, including 2-Hexadecen-1-ol, Nerolidol B (Cis or Trans), Heneicosan, Vitamin E, 3, 7, 11, and 15-Tetramethyl-, and [R-[R*,R*-(E)]]. Squalene, Cyclopropanemethanol, Gamma-Sitosterol, (-)-Caryophyllene Oxide, and Neophytadiene. The plant Melaleuca leucadendra (L) L. can be regarded as a source of nonpolar compounds with promise for the treatment of mastitis because of the significance and the wide range of biological activities documented in the literature on the compounds found in this study.
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