No abstract

Summary — This paper reports improvements on a previous technique of ours for producing chalkbrood disease in Apis mellifera under controlled conditions. Mummification reached almost 95% (the previous technique reached 70%) when fifth instar larvae were chilled at 18 °C 24 h before sealing and kept at 25 °C for 6 days after operculation. When the larvae were chilled, but the temperature after operculation was 30 or 35 °C, mummification reached 43.65 and 29%, respectively. Percentages of mummification were lower when chilling prior to sealing cells was not applied: 77.62% (at 25 °C after sealing), 15.31 % (at 30 °C) and 2.22% (at 35 °C). The effect of a high relative humidity (rh) (87%) combined with slight chilling (30 °C) induced a higher percentage of mummification (7.75%) then compared to the same temperature but lower rh (only 0.95% of larvae were mummified at 68% rh).