A fe r r u ge m a la r a n ja d a , c a u sa d a p or Pu c c i n i a k u e h n i i é a tu almente u ma da s doenças ma is importantes da cana-de-açúcar, Palavras-chave adicionais: Epidemiologia, incubação, condições ambientais. n a s t em pe ra tu ra s: 1 0 , 1 5 , 2 0 , 2 5 e 3 0°C , em sei s pe rí od os d e incu ba çã o: 1 , 3, 6 , 1 2 , 1 8 , 2 4 hora s, tota liza ndo 3 0 tra ta mentos c o m 5 re p etições. Ao fina l de ca da período, a germina çã o foi interrompida adicionando-se 0 ,1 ml de lactofenol. Dividiu-se cada placa em qu atro campos e a valiou-se em cada u m, 50 esporos. Os dados de porcentagem de germinação foram submetidos à análise de variância em esquema fatorial 5x6 com desdobramento da interação em polinômios ortogona is. Pelo a ju ste de modelo ma temá tico, a germinação máxima ocorreu para o período de 12h e à temperatura de 21°C (R 2 =82%). Para os períodos de incubação, o modelo estimou qu e na cu rva de tempera tu ra 2 0°C, fora m necessá ria s 1 4 hora s (R 2 =65%) para se atingir a germinação máxima.Keywords: Epidemiology, incubation, environmental conditions. ABSTRACTT he ora nge ru st ca used by Puc cinia k ue hn ii, is currently one of the most importa nt disea ses of su garca ne du e to the potentia l da ma ge to su scep tible va rieties. T his stu dy eva l u a ted the effect of tempera ture on germina tion of u redospores. Uredospores were collected in the commercial field of suga rcane, variety SP 8911 15 in the city of Andirá (PR). After being removed from lea ves, the u redospo res u nderwent sepa ra tion of imp u rities a nd pla ced in a solu tion of distilled water with Tween-20 . T he spores suspension was calibrated using a Neubauer chamber and pla ted an aliquot of 0.1 ml on the a gar-water (1.5%). The plates were placed in BOD, a t t em p er a tu r es: 1 0 , 1 5 , 2 0 , 2 5 a n d 3 0 ° C i n si x i nc u ba t io n Minchio, C. A.; Canteri, M.G.; Rocha, J.A. Germination of uredospores of Puccinia kuehnii subjected to different temperatures and incubation times. Summa Phytopathologica, v.37, n.4, p.211-214, 2011. pe riod s: 1 , 3 , 6 , 1 2 , 1 8 , 2 4 hou rs, tot a li ng 3 0 tr ea t ment s wi th fiv e re p l ic a t io n s. At t h e e n d o f e a ch p er i o d, ge r m in a t io n wa s stopped by adding 0 .1 ml of lactophenol. Each pla te wa s divided into four fields and 50 spores were assessed in each field. Data on germina tion percentage were subjected to a nalysis of variance in a fa ct ori a l 5 x6 wit h th e u nfol din g of in tera cti on in o rth ogon a l polynomia ls. By a djustment of ma thema tical model, the maximum germ ina tion occu r red for the pe riod of 1 2 h a t a tem pera tu r e of 21°C (R2 = 82%). For the incubation periods, the model estimated that in the curve of tempera ture 20°C were requ ired 14 hou rs (R2 = 6 5 %) to rea ch maximu m germina tion.A ferrugem alaranjada da cana-de-açúcar, cujo agente etiológico é o fungo Puccinia kuehnii (W. Krüger) E.J. Butler, é uma doença que está presente em diversas partes do mundo (5,12,14,16), sendo que o seu pr...
The aim of this study was to evaluate changes in the morfology and length of the germ tube of Puccinia kuehnii, under the influence of temperature and incubation time. The development of the germ tube was asssesed for 24 hours. The concentration of uredospores was 2x105 esporos.ml-1 calibrated by a Neubauer chamber and plated in a 0.1 ml aliquot in water – agar (1.5%), placed in BOD and regulated at temperatures of 10, 20 and 30 °C. Readings were performed after 1, 3, 6, 12, 18 and 24 hours, with 5 replications. At the end of each period, germination was stopped by adding 0.1 ml of lactophenol. The length and morphology of 10 germinated spores per plate were evaluated with the help of a Motic Images MCCamera – Plus 2.0 ML software. Data were subjected to analysis of variance in a 3x6 factorial design with an interaction in orthogonal polynomials. The model estimated that a temperature of 20 °C, at 24 hours of incubation, was required to reach the maximum length. Germ tubes morphological changes were observed at incubation temperatures of 10, 20 and 30 °C showing zigzag, rectilinear and branched forms, respectively.
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