Mosquitoes (Diptera: Culicidae) are vectors of important parasites and pathogens causing death, poverty and social disability worldwide. The overuse of synthetic insecticides to control mosquito vectors lead to resistance, adverse environmental effects and high operational costs. Therefore, the development of eco-friendly control tools is an important public health challenge. In this study, two different essential oils (EO) (geranium, Geranium maculatum, and bergamot, Citrus bergamia) loaded polymeric nanoparticle (PN) were elaborated using polyethylene glycol (PEG) and chitosan (Qx) as the polymeric matrix/coating. In addition, the mosquito larvicidal acute and residual activity of the PN was evaluated on Culex pipiens pipiens. The physicochemical characterization of PN revealed that PEG-PN had sizes <255 nm and encapsulation efficiency between 68 and 77%; Qx-PN showed sizes <535 nm and encapsulation efficiency between 22 and 38%. From the toxicological test, it was observed that Qx-PN produced higher acute and residual activity than PEG-PN. Overall, this study highlights that polymer nanoparticles containing essential oil are a promising source of eco-friendly mosquito larvicidal products.
Microemulsions (ME) are thermodynamically stable isotropic mixtures of oil, water, and surfactant; they would also be attractive as potential insecticidal products due to the high bioviability of the active ingredient, attributable to the small sizes of the oil drops. A laboratory study was conducted in order to compare the biological effect of oil in water (o/w) geranium essential oil (EO) and geraniol MEs and emulsions, against Culex pipiens pipiens mosquito larvae. The systems were based on three nonionic surfactants (Cremophor EL, Brij 35, Tween 80). The MEs showed dispersed phase diameters in the range of 8 to 14 nm and had low PDI values (<0.2). The MEs were analyzed by TEM, indicating that they had nearly spherical morphology. The microemulsified systems based on geranium EO and those of geraniol produced a notable increase of the larvicidal activity when compared with the respectably emulsions, concluding that the biological effect is related with the diameter of the dispersed phase. The smallest drops achieved the highest larvicidal activity, being the aqueous nanoformulations based on geraniol most effective than those of geranium EO. However, geranium microemulsions are preferred due to their residual toxicological profiles. The results indicate that these novel systems could be used in integrated pest management program for the C. pipiens pipiens.
The physicochemical characterization and residual insecticidal activity of poly(ethylene glycol) (PEG) nanoparticles containing essential oils (EOs) from geranium (Geranium sp.) and bergamot (Citrus reticulata L.) were evaluated against Blatella germanica for 1 year. The nanoparticles' size increased during the storage time from \235 to \450 nm; the EO content decreased approximately 50 %, and the abundance of the major components did not show any differences between pre-and post-formulation. The surface characteristics of nanoparticles were analyzed by transmission electronic microscopy. The EO nanoparticles produced a notable increase in the residual contact toxicity apparently because of the slow and persistent release of the active terpenes. In addition, the nanoformulation enhanced the EO contact toxicity. The results indicate that these novel systems could be developed as control agents against German cockroaches.
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