Las algas pardas constituyen una fuente de alto contenido de polisacáridos como los fucoidanos que poseen importantes propiedades inmunomoduladoras. El objetivo fue determinar la viabilidad de células mononucleares de sangre periférica humana (CMSPh), producción de óxido nítrico (NO), especies reactivas de oxígeno (ROS) y de las citoquinas proinflamatorias IL-1α, IL-6, TNF-α e IFN-γ en cultivos tratados con fucoidan de Lessonia trabeculata. Se empleó fucoidan de Lessonia trabeculata proveniente de la bahía San Nicolás de Marcona-Ica. Las CMSPh se aislaron empleando Ficoll-Hypaque, se distribuyeron a una concentración de 1x105 células/pocillo en medio RPMI-1640 completo y se trataron con diferentes concentraciones de fucoidan durante 24 y 48 h. La actividad citotóxica se determinó por la reducción de MTT, la producción de NO por la reacción de Griess y las ROS por la reducción del NBT. La producción de citoquinas se cuantificó por ELISA. El fucoidan de L. trabeculata estimuló la proliferación de CMSPh y produjo el incremento de ROS a concentraciones de 100-2000 μg/mL respecto al control (p<0.001), la reacción para nitritos resultó negativa. El fucoidan incrementó la producción de IL-1α y TNF-α a concentraciones de 100 y 10 μg/mL respectivamente, mientras que la producción de IL-6 e IFN-γ no mostró diferencias significativas. Se concluye que el fucoidan de L. trabeculata estimula la proliferación de CMSPh, producción de especies reactivas de oxígeno y las citoquinas proinflamatorias IL-1α y TNF-α que poseen importantes propiedades inmunomoduladoras.
No abstract
Pitahaya has originated worldwide interest due to its content of bioactive compounds with proven beneficial effects on health, acting as antioxidants against free radicals. This study aimed to evaluate the nutraceutical potential of the peel and pulp of the red (Hylocereus monacanthus) and yellow (Hylocereus megalanthus) pitahaya ecotypes for nutritional formulation purposes. Two pitahaya ecotypes were analyzed, obtaining a methanolic extract of the peel and edible part to perform the proximal chemical analysis, the phytochemical screening, and determine antioxidant activity by the DPPH, ABTS, and IC50 methods. Flavonoids, tannins, quinones, among other bioactive compounds were identified. Yellow pitahaya presented higher content of polyphenols and higher antioxidant activity by the ABTS method, while the average inhibition percentage for both ecotypes was 93% by DPPH method. IC50 was higher for the edible part of red pitahaya with 1.68 mg mL-1. Both ecotypes have a high content of polyphenols and a high antioxidant capacity, which agree with those found in different studies such as those of Colombia, Brazil and Korea, being as high or even higher than most varieties of citrus fruits in Peru. Future studies should consider the inclusion of other metabolites and bioactive substances such as betalains due to their antioxidant activity. Both pitahaya ecotypes are rich in antioxidants, bioactive compounds, have low energy density, and may be suitable for food prescriptions as a functional ingredient in food industry.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
334 Leonard St
Brooklyn, NY 11211
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.