This study was conducted to investigate the association of Ghrelin gene polymorphisms and expression levels with some biochemical traits of broiler chickens. Two hundred broiler chicks Ross308, one-day old were wing-tagged and reared under optimal conditions. Blood samples were collected individually from all birds on 21 and 35 days of age. Proventriculus was collected at 35 days of age from thirty birds of each of groups sorted according to growth rate for high, moderate and low to measure ghrelin gene expression by real-time RT-PCR. The serum traits of glucose, uric acid, cholesterol, triglyceride, high density lipoproteins (HDL), low density lipoproteins LDL, total protein, albumin, globulin, aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP) were not influenced by ghrelin gene polymorphisms. While significant (P < 0.01) differences were recorded among the three chicken groups with high, moderate and low ghrelin expression in serum glucose, uric acid, total protein, albumin, cholesterol, triglyceride, HDL, AST, ALT and ALP concentrations at 21 and 35 days of age which increased with increasing ghrelin gene expression. The PCR- RFLP technique revealed no significant differences between GG and LL genotypes of ghrelin gene in all biochemical traits at 21 and 35 days of age, whereas ghrelin gene expression had a significant effect on serum glucose, uric acid, total protein, albumin, cholesterol, triglyceride, HDL, AST,ALT and ALP.
This experiment was conducted to study the association of Ghrelin gene polymorphisms and expression levels with some productive and carcass traits of broiler chickens. Two hundred broiler chicks, one day old Ross308, were wing-tagged and reared under conventional conditions. Blood samples were collected individually of all birds to study of ghrelin gene polymorphisms by PCR-RFLP technique. Proventriculus was collected at 35 days of age from thirty birds of each groups sorted according to high, moderate and low growth rate to measure ghrelin gene expression by real-time RT-PCR. Result revealed that final body weight and weight gain were not significantly influenced by ghrelin gene , whereas body weight and weight gain of males and females with high ghrelin gene expression were significantly (p<0.01) higher than those of moderate and low ghrelin gene expression at 21 and 35 days of age. Ghrelin gene polymorphisms had no significant (p>0.05) influence on carcass traits and edible organ weights, while significant (P < 0.01) differences were existed among high, moderate and low ghrelin gene expression groups in carcass weight, breast, wing, neck and back relative weights. It is concluded that PCR- RFLP technique revealed no significant effect for ghrelin gene polymorphisms on productive and carcass traits, whereas ghrelin gene expression had significant effect on productive traits.
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