Rock bream iridovirus (RBIV) causes huge losses, especially in rock bream Oplegnathus fasciatus. Rock bream injected with RBIV and held at 29, 26, 23 or 20 °C had 100% mortality. Conversely, all infected fish held at 17 °C survived even after the temperature was progressively increased to 26 °C at 100 dpi. Rock bream exposed to virus and held for 2, 4 and 7 days at 23/26 °C before the temperature was reduced to 17 °C had mortality rates of 26.6/73.2%, 66.6/100% and 93.4/100%, respectively, through 100 dpi. When surviving fish had the water temperature increased from 17 to 26 °C at 100 dpi, they did not exhibit signs of disease and had low virus copy numbers (below 10(3)). To investigate the development of a protective immune, rock bream were infected with RBIV and held at 23 °C before shifting the water temperature to 17 °C at 4 dpi. All injected fish survived until 120 dpi. While 100% of the previously unexposed fish died, 80.2% of the previously infected fish survived. When the survivors were rechallenged again at 160 dpi, no further mortality occurred. The high survival rate of fish following rechallenge with RBIV indicates that protective immunity was established in the surviving rock bream.

Grateloupia elliptica (Holmes, 1896) is a red alga belonging to the order Halymeniales and phylum Rhodophyta. In this study, the complete mitochondrial DNA (mtDNA) of G. elliptica has been described. The complete circular mtDNA of G. elliptica was 28,503 bp in length, with an A + T content of 68.78%; it encoded a total of 49 genes, including 20 tRNA, three rRNA, and 26 protein-coding ( CDS ) genes. Phylogenetic analysis based on complete mitochondrial genomes revealed that G. elliptica was most closely related to G. angusta . The complete mitochondrial sequence of G. elliptica will enrich the mitochondrial genome database and provide useful resources for population genetics and evolution analyses.

Polyopes affinis ((Harvey) Kawaguchi & Wang, 2002) is a red alga in the order Halymeniales of the phylum Rhodophyta. The entire mitogenome of P. affinis was sequenced and compared to related Halymeniales species. The entire circular-mitogenome is 25,988 bp long, has 27.59% GC content, and comprises 25 protein-coding genes (CDS), 23 transfer RNA (tRNA) genes, and three ribosomal RNA (rRNA) genes. In terms of gene synteny and tRNA composition, the P. affinis mitogenome differs significantly from that of P. lancifolius . Phylogenetic analysis shows P. affinis mitogenome in a branch sister to P. lancifolius , indicating a close relationship with other Halymeniales species.

Prediction of quantity and quality of gas produced after landfilling is not easy because it is strongly dependent on the hydrological and biological parameters. The objective of this study was to develop the LEAGA-I model by combining a hydrological module of unsaturated flow theory with a biological decomposition module in order to predict methane gas production, even carbon dioxide gas production, using a pilot scale lysimeter. For a biological module, especially, three-step procedures, i.e., hydrolysis of refuse, acid formation, and methane formation were applied. As a result of this study, the LEAGA-1 model, developed by combining a hydrological module with a biological module, not only provided more reasonable prediction of gas production during landfill stabilization, but also turned out to be a useful model, as a result of comparison of the simulated values with the observed values of lab-reactor.

The development of LAMP primers based on genetic information from a public database, not virus-infected samples, may provide a very simple and convenient method to identify viral infection in aquatic organisms.