Microarray technology provides a unique tool for the determination of gene expression at the level of messenger RNA (mRNA). This study, the mRNA expression profiles provide insight into the mechanism of action of cadmium in Fleshy shrimp (Fenneropenaeus chinensis). The ability of genomic technologies was contributed decisively to development of new molecular biomarkers and to the determination of new possible gene targets. Also, it can be approach for monitoring of trace metal using oligo-chip microarray-based in potential model marine user level organisms.15K oligo-chip for F. chinensis that include mostly unique sets of genes from cDNA sequences was developed. A total of 13,971 spots (1,181 mRNAs up-regulated and 996 down regulated) were identified to be significantly expressed on microarray by hierarchical clustering of genes after exposure to cadmium for different conditions (Cd24-5000 and Cd48-1000). Most of the changes of mRNA expression were observed at the long time and low concentration exposure of Cd48-1000. But, gene ontology analysis (GO annotation) were no significant different between experiments groups. It was observed that mRNA expression of main genes involved in metabolism, cell component, molecular binding and catalytic function. It was suggested that cadmium inhibited metabolism and growth of F. chinensis .
Through the study of Bohenggyuljangbuyongyakbubyo(輔行訣臟腑用藥法要) focusing on the newly illuminated subject 'The study of Donhuang(敦煌學)' of China, the author analyzed and researched DonhuangzhongyiyakjunseoㆍBohenggyuljangbuyongyakbubyo(敦煌中醫藥全書ㆍ輔行訣臟腑用 藥法要) in order to study and report the origin of prescriptions from Sanghanjabbyungron(傷寒雜病 論) , which was written by Zhangzhongjing(張仲景).Among around 50 prescriptions included in Bohenggyul, 18 prescriptions similar to Sanghanjabbyungron could be classified in five different manners as follows. 1) There are nine examples whose prescription composition and chief virtue are same but names different.2) There are five examples whose prescription composition is same but chief virtue and names different.3) There are two examples whose names are same but prescription composition and chief virtue different.4) There is one example of prescription as a combination of two or more prescriptions. 5) There is one example whose prescription composition and possible variations of prescription are same but chief virtue different.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.