Several published censuses have noted the presence of two tyrannosaurids, Daspletosaurus sp. and Albertosaurus sarcophagus, within the Upper Cretaceous Horseshoe Canyon Formation of Alberta. Although A. sarcophagus is known from more than a dozen major discoveries in these strata, Daspletosaurus sp. is known from just a single problematic skeleton (lacking most of the skull) of a young individual. Here we describe and figure this skeleton, and marshal a variety of osteohistologic, morphometric, and phylogenetic methods to accurately determine its taxonomic status. Although none of these methods individually provides convincing evidence regarding the affinities of the specimen, together (and including other historical and biostratigraphic considerations) they strongly imply that the skeleton instead pertains to a young A. sarcophagus. In this way, we show that only a single species of tyrannosaurid is definitively present in the Horseshoe Canyon Formation, greatly simplifying interpretations of tyrannosaurid evolution and ecology in this setting. Anat Rec, 303:673–690, 2020. © 2019 The Authors. The Anatomical Record published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.