The use of dried blood spots on filter paper has been shown to be a practical alternative in several studies with humans and animals, enabling a simple means of storing and transporting viable blood samples for various laboratory analyses. However, its applicability in the measurement of progesterone in animals is scarce. Thus, the objective of this study was to evaluate the feasibility of using dried blood spots for the measurement of progesterone in sheep. In total, 38 blood samples from 6 sheep were dripped onto filter paper, and the remainder of each sample was separated into serum. The progesterone levels in the serum samples and in the dry drops were measured by enzyme immunoassay and subsequently correlated. The levels of progesterone in the serum and dry spots showed a high correlation between the matrices (R2 = 0.9694). In conclusion, this study demonstrated the feasibility of using samples of dried sheep blood spots for the measurement of progesterone, and the storage and transport technique can be applied in the field.
Resumo. O objetivo deste estudo foi avaliar os níveis de metabólitos fecais de progesterona e estradiol ao longo do ciclo estral em fêmeas suínas. Assim, amostras de fezes foram coletadas de quatro fêmeas adultas saudáveis e vazias duas vezes por semana por 75 a 200 dias, dependendo do indivíduo. Os metabólitos fecais de progesterona e estradiol foram dosados por enzimaimunensaio. A duração do ciclo estral estimada (~21 dias), bem como a duração das fases progesterônica (~12 dias) e não progesterônica (~8 dias) corroboraram com as relatadas na literatura, com diferenças entre as fases para os metabólitos fecais de progesterona. Houve uma ausência de sincronia entre os metabólitos fecais de progesterona e estradiol, relacionada com as diferenças na metabolização e excreção destes hormônios. Este estudo demonstrou a viabilidade no uso de amostras fecais para o monitoramento longitudinal do ciclo estral em suínos. Entretanto, é necessário considerar as diferenças na metabolização dos hormônios ao avaliar a interação hormonal durante o ciclo estral.
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