In this study, the kinetic isotope effects and solvent isotope effects in the reaction of the deamination of [(1R)-(2)H ] putrescine--catalysed by enzyme diamine oxidase (EC 1.4.3.6)--were determined using a non-competitive spectroscopic method. Putrescine, stereospecifically labelled with deuterium, was obtained by enzymatic decarboxylation of l-ornithine that was carried out in a fully deuteriated incubation medium.
Since the refugee crisis of 2015, European institutions and governments strengthened policies to better manage migration flows and protect EU’s external borders. In the external dimension, the Union implemented a wide variety of economic, political and deterrence measurers to regain control over migratory flows. Though development cooperation was declared one of important tools for addressing root causes of migration, the externalization of migration management to neighboring transit countries became the main pillar of anti-crisis strategy. Although this policy enabled to essentially reduce the number of irregular arrivals to Europe, it cannot be considered as a long-term solution, as recent developments on Greece-Turkey border reminded. To be better prepared for migration challenges of the future the EU should rethink its development cooperation with the origin and transit countries and include both forced and economic migrants in its comprehensive response. Aid can be a useful tool for the EU if it is used to manage rather than to stop migration.
The economic crisis showed the weaknesses of the institutional system of the European Union, raising questions about its shape. The article aims to determine whether internal structures of the EU institutions and their modes of operation are chances or barriers for effective treaty implementation and the ability of institutions to face unexpected, difficult situations. The article focuses both on the formal structure of the institutions and their human resources. The article aims to conclude if the crisis may give impetus to a significant improvement in the EU institutional system.
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