Mitochondrial DNA restriction site analyses on natural populations of Drosophila subobscura have proved the existence of two common, coexisting haplotypes (I and II), as well as a set of less frequent ones derived from them. To explain this distribution, experiments to date point practically to all possible genetic mechanisms being involved in the changes of gene frequencies (cytonuclear coadaptation, direct natural selection on mtDNA and genetic drift). In an attempt to find differences that help to understand the dynamics of these haplotypes and to detect the effect of selection, we measured certain fitness components and life-history traits (egg-larva and larva-adult viabilities and developmental times, longevity, resistance to desiccation and optimal density) of the two main haplotypes I and II when maintained in laboratory population cages. As a general trend, haplotype II showed a higher net fitness than haplotype I, which explains the superiority of haplotype II over haplotype I in experimental populations but not their coexistence in nature, where additional factors must be considered.

We tested different fitness components on a series of conspecific mtDNA haplotypes, detected by RFLPs in Drosophila subobscura. Additionally, haplotype VIII, endemic to the Canary Islands, was tested upon its own native nuclear DNA background and upon that of the rest of mtDNAs tested herein. We found that both nuclear and mitochondrial DNA can have a significant effect upon their hosts' fitness, and that negative selection is one of the mechanisms that can intervene in this species' mtDNA haplotype pattern. We discuss the importance of this mechanism in relation to genetic drift, in the form of periodic population bottlenecks, and how the latter can enhance the former. We also detected a significant positive effect of haplotype VIII upon fitness that could explain in part the dominance of this endemic haplotype on some of the Canary Islands, and a mitochondrial heterosis involving this haplotype when on a foreign nuclear DNA background.

The mating pattern and female fertility on the two main mitochondrial DNA haplotypes (I and II) of Drosophila subobscura were studied, in an attempt to find possible differences between them in relation to sexual selection or isolation that could explain the populational dynamics and the co-existence of these two strains in nature. The mating pattern indicated an assortative mating in population cages, where couples of the same haplotype, mainly those of haplotype I, mated more often. However, the significations detected in laboratory conditions disappeared in wild populations, where random mating was the rule. The female fertility also showed differences in the laboratory compared to the wild, since couples with haplotype I males were more efficient in the laboratory populations. These results, together with others that we previously obtained, either point to selection acting directly on the mtDNA or to the presence of some kind of cytonuclear co-adaptation in these two haplotypes, although this must be modulated by other factors that change with the seasons and time. The end result could well be a balance of opposite forces acting on both haplotypes.

Restriction site analysis of mitochondrial DNA (mtDNA) was carried out on 607 isofemale lines, corresponding to monthly samples obtained over a two-year period, from a single geographic population of Drosophila subobscura to evaluate the possible changes in the action of the evolutionary forces with respect to the variation of the seasonal enviromental conditions. The haplotype distribution pattern was: (1) two highly frequent haplotypes and (2) sporadic haplotypes that were almost never found again after a population bottleneck; similar to that observed for the entire range of this species on the European continent. We detected significant negative D-values for the entire population, reflecting an excess of rare haplotypes. The results confirm that the coexistence of the main haplotypes in nature, and the rare ones, could be mainly caused by seasonal population expansions. However, the fact that the frequencies of the two major haplotypes are exchanged in some months, could indicate that the action of selection cannot be discarded, possibly acting upon gene arrangement associated with mtDNA haplotypes.

With the use of a greater variety of metals and methods of finishing them, an increasing need to measure metallic appearance has developed in the automotive industry. A simple and easy to operate instrument has been designed to measure the geometric characteristics of reflectance related to metallic appearance. These are specular reflectance, distinctness of image, haze, and diffuseness. A series of selected aluminum and stainless steel specimens has been used to test the performance of the new instrument and of older devices with which it has been compared. Functionally, the new instrument combines features of the Distinctness of ReflectedImage (DORI)meter designed by Tingle, and the abridged goniophotometer designed by Tingle and George. The design and operation of the new instrument have been simplified by use of multiple receptor apertures with optical fiber light collectors. The measurement of a wide range of metal appearance characteristic has thus been achieved with mechanical and electrical circuit simplicity.

The standard measurement situation for color involves a specimen which is flat, uniform in color, and thin. For measurements of colors of many food products, instruments which give reliable and reproducible results when applied to the flat, uniform and thin traditional specimens are unreliable. This is because specimens of foodstuffs come in a variety of shapes, sizes and translucencies. The unreliability of instrument measurements can be shown to be related to instrument differences in geometry and to: (a) specimen nonflatness; (b) specimen variability in pattern, particle size, shape, compression, and/or humidity; and (c) specimen translucency.

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