An acidophilic sporeformer was isolated from several varieties of shelf-stable juices. The organism sporulated on potato dextrose agar (pH 3.5) at 37°C within 24 hours and grew well in fruit and berry juices. The pH range of growth in potato dextrose broth was 3.0 to 5.3. The D87.8°C' D91.1°C' and D95°C determined in berry juice were 11.0, 3.8, and 1.0 min, respectively. The ability of this organism to grow at low pH and to survive pasteurization poses a threat of economic loss by spoilage to beverage producers. The organism could not be identified based on its characteristics or the fatty acid profile comparison to those of other Bacillus species, including the acidophilic B. acidocaldarius.

Salmonella in eggs and egg products with fluorescent antibody. Appl. Microbiol. 12:447-450. 1964.-Organisms of the genus Salmonella are detected in eggs and egg products within 24 hr in the presence of Pseudomonadaceae and other Enterobacteriaceae by combining selective cultural methods with fluorescentantibody techniques. These techniques are specific for Salmonella when H antibodies are used. Absorption techniques are necessary before the 0 antibodies give specific reactions for Salmonella. No cross-reactions appear when H antiserum is used. Absorption and interference techniques indicate the test is specific for Salmonella. Salmonellosis is one of the important food-borne infections in the world today; the United States alone reported almost 19,000 cases in 1963 (U.S. Department of Health, Education, and Welfare, 1964). Conventional methods for the isolation and identification of Salmonella are tedious and time-consuming. The development of a rapid and accurate test would be an invaluable aid toward controlling these organisms in dried eggs and egg products. Coons et al. (1942) introduced the fluorescent-antibody (FA) technique and made possible a new and rapid serological method of detecting microorganisms. In principle, this technique is an immunological staining procedure which uses homologous antibody conjugated with suitable fluorochromes. FA technique is now a common tool in microbiology and has generally been proved satisfactory. Thomason, Cherry, and Moody (1957) applied this method for the detection of several serotypes of Salmonella in pure culture. Later, Thomason, Cherry, and Edwards (1959) found that the value of the method for the rapid detection of Salmonella in feces was impaired by nonspecific staining.

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Organisms of the genus Salmonella are detected in eggs and egg products within 24 hr in the presence of Pseudomonadaceae and other Enterobacteriaceae by combining selective cultural methods with fluorescent-antibody techniques. These techniques are specific for Salmonella when H antibodies are used. Absorption techniques are necessary before the O antibodies give specific reactions for Salmonella . No cross-reactions appear when H antiserum is used. Absorption and interference techniques indicate the test is specific for Salmonella .