The results of the study are presented clearly, honestly, and without fabrication, falsification, or inappropriate data manipulation. The results of the present study do not constitute endorsement by the American College of Sports Medicine.

Acute glutamine supplementation does not improve 20-km self-paced cycling performance in the heat.

Data from this study suggest not all athletic subgroups require a familiarisation to produce substantially reliable 10 and 20km cycling performance. However, a familiarisation considerably improves the reliability of pacing strategy adopted by recreational runners and team sport athletes across these distances.