Although optogenetics has revolutionized rodent neuroscience, it is still rarely used in other model organisms as the efficiencies of viral gene transfer differ between species and comprehensive viral transduction studies are rare. However, for comparative research, birds offer valuable model organisms as they have excellent visual and cognitive capabilities. Therefore, the following study establishes optogenetics in pigeons on histological, physiological, and behavioral levels. We show that AAV1 is the most efficient viral vector in various brain regions and leads to extensive anterograde and retrograde ChR2 expression when combined with the CAG promoter. Furthermore, transient optical stimulation of ChR2 expressing cells in the entopallium decreases pigeons’ contrast sensitivity during a grayscale discrimination task. This finding demonstrates causal evidence for the involvement of the entopallium in contrast perception as well as a proof of principle for optogenetics in pigeons and provides the groundwork for various other methods that rely on viral gene transfer in birds.

Trigeminal ganglion and sensory nerves suggest tactile specialization of elephants Highlights d The elephant trigeminal ganglia are larger than a macaque monkey brain d The elephant infraorbital nerve innervating the trunk contains 4,00,000 axons d The elephant infraorbital nerve is thicker than all other sensory nerves d Elephants might be very tactile animals

Summary Multi-component behavior is a form of goal-directed behavior that depends on the ability to execute various responses in a precise temporal order. Even though this function is vital for any species, little is known about how non-mammalian species accomplish such behavior and what the underlying neural mechanisms are. We show that humans and a non-mammalian species (pigeons) perform equally well in multi-component behavior and provide a validated experimental approach useful for cross-species comparisons. Applying molecular imaging methods, we identified brain regions most important for the examined behavioral dynamics in pigeons. Especially activity in the nidopallium intermedium medialis pars laterale (NIML) was specific to multi-component behavior since only activity in NIML was predictive for behavioral efficiency. The data suggest that NIML is important for hierarchical processing during goal-directed behavior and shares functional characteristics with the human inferior frontal gyrus in multi-component behavior.