Rats were subjected to cardiac arrest and resuscitation, 90 min of reperfusion, and in situ perfusion fixation. Thiobarbituric acid (TBA) was included in the aldehyde-free perfusion fixative, the TBA reaction was driven in situ by heating, and fluorescence microscopy was utilized to characterize the location of products of the TBA reaction. Absorbance-difference spectra were performed on butanol-extracted brain homogenates to confirm in situ formation of TBA adducts with aldehydic products of lipid peroxidation. Nissl-stained sections revealed good cellular fixation without shrinkage artifacts. Fluorescence was not seen microscopically when TBA was omitted from the perfusion fixative, and little fluorescence was present in normal brains or brains after ischemia only. However, after 90-min reperfusion, intense granular fluorescence was seen in the neuronal perikarya (especially at the base of the apical dendrite) of numerous pyramidal neurons in cortical layers 5 and 6 and in the pyramidal layer of Ammon's horn in the hippocampus. The nuclei of these cells exhibited no fluorescence. Fluorescence was also present in some striatal neurons, but was absent in the adjacent radial bundles. Neither glia nor white matter exhibited similar fluorescence. These observations indicate that neurons in the selectively vulnerable zones of the cortex and hippocampus are early and specific targets of lipid peroxidation during post-ischemic reperfusion.

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Summary Effect of intramuscular administration (10 mg/kg/week) of testosterone and oxandrolone on a) thrombocyte aggregation and b) synthesis of prostaglandins from [14C]‐arachidonic acid in thrombocytes and aorta of atherosclerosis‐susceptible White Carneau pigeon was examined. Neither testosterone nor oxandrolone influenced collagen, ADP and arachidonic acid induced aggregation or the synthesis of prostaglandins in thrombocytes. However, both testosterone and oxandrolone stimulated (p < 0.05) the synthesis of 6‐keto‐PGF1α (stable product of prostacyclin) und PGE2 in aorta. Der Einfluß von Testosteron und Oxandrolon auf die Thrombocytenaggregation und die Prostaglandinsynthese in Thrombocyten und Aorta bei Atherosklerose‐disponierten Tauben Zusammenfassung Der Einfluß von Testosteron und Oxandrolon nach intramuskulärer Applikation (10 mg/kg/Woche) wurde sowohl a) auf die Thrombocytenaggregation als auch b) auf die Synthese von Prostaglandinen aus (14C)‐Arachidonsäure in Thrombocyten und Aorta bei Atherosklerose‐disponierten weißen Carneau Tauben untersucht. Dabei ergab sich, daß weder Testosteron noch Oxandrolon die durch Kollagen, ADP und Arachidonsäure induzierte Aggregation oder Synthese von Prostaglandinen in Thrombocyten beeinflußten. Dagegen stimulierten beide, Testosteron und Oxandrolon, signifikant (p < 0.05) die Synthese von 6‐keto‐PGF1α (fester Bestandteil von Prostacyclin) und PGE2 in der Aorta.