A protocol was developed for in vitro clonal propagation of Rauvolfia serpentina through direct regeneration from shoot tip explants. Multiple shoots (eight shoots per explant) induction were obtained on MS medium supplemented with 4.0 mg/l BAP and 0.5 mg/l NAA within 10-15 days. The elongated shoots rooted well in half strength MS medium with 0.5 mg/l NAA. The in vitro raised plantlets were acclimatized in glass house and successfully transplanted to field condition with 80 % survival. The results indicated that large scale commercial micropropagation of Rauvolfia serpentina is technically feasible.
Bangladesh J. Sci. Ind. Res. 42(1), 37-44, 2007
An efficient protocol was established for rapid and large scale propagation of woody aromatic medicinal plant Vitex negundo L. by in vitro shoot multiplication from shoot tips and nodal segments of mature plant. Of the four different growth regulators BA, Kn, GA 3 , NAA and coconut water, MS fortified with BA 1.0 mg/l was found to be the most effective for inducing multiple shoots from nodal explants. The percentage (96%) of shoot multiplication per node (21.83) was highest up to second subculture passages, after which there was a gradual decline in shoot development. Best rooting was induced (93%) in excised shoots on half strength MS medium supplemented with an optimal combination of NAA (0.3 mg/l). Soil, compost and sand (1:1:1) mixture was the most suitable planting substrate for hardening. The survival rate was 80% and the regenerated plants were successfully transferred to the soil.
Large scale Spirulina culture is possible in Bangladesh inspite of unpredictable climatic condition. Effect of different culture media on the growth of a local strain of Spirulina (Spirulina platensis) was studied. Bangladesh medium (Bd1) was found to be more favourable for the growth of the alga. All together three culture media were included in this study.
Bangladesh J. Sci. Ind. Res. 41(3-4), 227-234, 2006
Spirulina containing high concentration of functional nutrients is emerging as an important therapeutic food. So the present study was designed to evaluate the hypoglycaemic properties of Spirulina platensis on long-evans rats. Two sets of experiment were conducted, the 1st set was compared with the effect of Spirulina platensis, glibenclamide, glucose and water after feeding 60 minutes. Glibenclamide was used as a standard reference drug. The findings clearly indicated that the oral administration of Spirulina platensis (150mg/kg b.w) significantly tended to reduce blood glucose level of rat than that of glibenclamide. In the 2nd experiment long-term hypoglycaemic effect of Spirulina platensis 0.5% and 2.5% fed with high fat high sugar containing diet and 150mg/kg b.w with normal laboratory diet was also observed. The 2nd findings also indicated that the blood glucose level was significantly decreased in both the diet when supplemented with Spirulina platensis. Key words: Diabetic mellitus; High fat diet; Hypoglycaemic; Spirulina platensis; Body weight DOI: 10.3329/bjsir.v45i2.5715Bangladesh J. Sci. Ind. Res. 45(2), 163-168, 2010
A protocol was established for mass propagation of a valuable medicinal herb, Eclipta alba (Linn.) Hassk (Asteraceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mgl -1 BAP + 0.1 mgl -1 NAA, in which 94% of the explants produced 18 shoots per culture.Repeated subcultures in the same medium, resulted rapid shoot multiplication with 26 shoots per culture. In vitro raised shoots rooted on half strength MS medium with 1.0 mgl -1 IBA +1.0 mgl -1 NAA.For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks.The survival rate of regenerated plantlets was 80%.
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