Characterizing the current population structure of potentially invasive species provides a critical context for identifying source populations and for understanding why invasions are successful. Non-native populations inevitably lose genetic diversity during initial colonization events, but subsequent admixture among independently introduced lineages may increase both genetic variation and adaptive potential. Here we characterize the population structure of the gypsy moth (Lymantria dispar Linnaeus), one of the world's most destructive forest pests. Native to Eurasia and recently introduced to North America, the current distribution of gypsy moth includes forests throughout the temperate region of the northern hemisphere. Analyses of microsatellite loci and mitochondrial DNA sequences for 1738 individuals identified four genetic clusters within L. dispar. Three of these clusters correspond to the three named subspecies; North American populations represent a distinct fourth cluster, presumably a consequence of the population bottleneck and allele frequency change that accompanied introduction. We find no evidence that admixture has been an important catalyst of the successful invasion and range expansion in North America. However, we do find evidence of ongoing hybridization between subspecies and increased genetic variation in gypsy moth populations from Eastern Asia, populations that now pose a threat of further human-mediated introductions. Finally, we show that current patterns of variation can be explained in terms of climate and habitat changes during the Pleistocene, a time when temperate forests expanded and contracted. Deeply diverged matrilines in Europe imply that gypsy moths have been there for a long time and are not recent arrivals from Asia.

The anaerobic decomposition of particulate organic tnatter (POM) was examined in the anoxic pelagic sediments of hypereutrophic Wintergreen Lake. Degradation of sedimented POM occurred rapidly as shown by increased production and release of ammonia, hydrogen sulphide, volatile fatty acids and methane from the sediments 2-3 weeks after large inputs of organic matter. Maximum concentrations of most metabolites were found at the sediment-water interface, indicating that the initial anaerobic degradation of freshly deposited POM occurred at this site. The absence of the inorganic electron acceptors, nitrate and sulphate, suggested that fermentation and methanogenesis were the major anaerobic processes involved in the dissimilation of organic matter in these sediments during stratified periods. The amount of carbon input converted to methane in the sediments was determined from May to early November 1976 and 1977. Carbon output as methane was measured by quantifying methane lost from the sediments by ebullition and by estimating soluble methane lost to the water column by diffusion. Total methane release during summer stratification accounted for 34% of the particulate organic carbon input to the sediments in 1976 and 44^/^ in 1977. Methane release was directly related to the rate of sedimentation of POM. However, methane production was temporarily inhibited following high rates of sedimentation in 1976, suggesting that the rate of organic loading may be an important factor controlling anaerobic decomposition in these sediments.

Global trade facilitates the inadvertent movement of insect pests and subsequent establishment of populations outside their native ranges. Despite phytosanitary measures, nonnative insects arrive at United States (U.S.) ports of entry as larvae in solid wood packaging material (SWPM). Identification of wood-boring larval insects is important for pest risk analysis and management, but is difficult beyond family level due to highly conserved morphology. Therefore, we integrated DNA barcoding and rearing of larvae to identify wood-boring insects in SWPM. From 2012 to 2015, we obtained larvae of 338 longhorned beetles (Cerambycidae) and 38 metallic wood boring beetles (Buprestidae) intercepted in SWPM associated with imported products at six U.S. ports. We identified 265 specimens to species or genus using DNA barcodes. Ninety-three larvae were reared to adults and identified morphologically. No conflict was found between the two approaches, which together identified 275 cerambycids (23 genera) and 16 buprestids (4 genera). Our integrated approach confirmed novel DNA barcodes for seven species (10 specimens) of woodborers not in public databases. This study demonstrates the utility of DNA barcoding as a tool for regulatory agencies. We provide important documentation of potential beetle pests that may cross country borders through the SWPM pathway.

No abstract

SUMMARY. Sedimenting seston was collected in May‐November 1976 and 1977 in sedimentation traps anchored 0.5 m above the pelagic sediments of hypereutrophic Wintergreen Lake. Sedimentation rates differed greatly in the two years studied, ranging from 2.7 to 19.3 g m−2 day−1 in 1976 and from 2.6 to 9.5 g m−2 day−1 in 1977. Because of the shallowness of the lake (maximum depth 6.5 m), the quantity and quality of sedimenting particulate organic matter (POM) was closely linked to the production dynamics of the phytoplankton. Chemical analyses indicated that the sedimenting POM was planktonic in origin and, in contrast to that of many deeper lakes, was dominated by protein. Short sedimentation distances coupled with the close proximity of the anaerobic hypolimnion to the photic zone ensured that the majority of sedimenting POM reached the sediments in a relatively undegraded form.

Automated instrumentation is described that performs time‐course incubation experiments directly in situ where natural conditions of temperature, light, hydrostatic pressure, etc. can be maintained. The sampler incubation device (SID) takes a 1‐liter sample from the water and simultaneously introduces an appropriate radiotracer. During subsequent in situ incubation, 50‐ml subsamples are withdrawn from the main sample at equally spaced intervals and preserved for laboratory analysis. Representative experiments revealed nonlinear carbon uptake within 0.5–1.0 h, emphasizing that even brief end‐point analyses can lead to large errors in estimating phytoplankton production rates. Studies of the rapid fluctuation in phytoplankton activity resulting from cloud‐induced variations in light intensity and the application of cellular fractionation methods for measuring the intracellular distribution of newly fixed carbon illustrated the utility of instrumental time‐course techniques for studying phytoplankton physiology and community metabolism in situ.

Strict anaerobic culture techniques were used to quantitatively and qualitatively evaluate the anaerobic heterotrophic bacteria present at the sedimentwater interface of hypereutrophic Wintergreen Lake (Augusta, Mich.). Anaerobic plate counts remained constant from March through December, 1973, ranging from 2.4 x 106 to 5.7 x 106 organisms/g (dry weight) of sediment. The isolatable bacteria represented a small percentage of the total microbial community, which was shown by direct microscopic counts to be 2.0 x 1011 organisms/g (dry weight) of sediment during June and July. Bacteria of the genus Clostridium dominated the isolates obtained, accounting for 71.8% of the 960 isolates examined. A single species, Clostridium bifermentens, comprised 47.7% of the total. Additional bacterial groups and the percentage in which they were isolated included: Streptococcus sp. (10.8%), unidentified curved rods (9.5%), gram-positive nonsporing rods (5.6%), and motile gram-negative rods (1.9%). Temperature growth studies demonstrated the ability of all the isolates to grow at in situ sediment temperatures. Gas-liquid radiochromatography was used to determine the soluble metabolic end products produced from [U-14C]glucose and a U-14C-labeled amino acid mixture by representative sedimentary clostridial isolates and by natural sediment microbial communities. At in situ temperatures the natural sediment microflora produced soluble fermentative end products characteristic of those elaborated by the clostridial isolates tested. These results are considered strong presumptive evidence that clostridia are actively metabolizing in the sediments of Wintergreen Lake.

European gypsy moth populations (Lymantria dispar L.) are well established and a proven destructive force in hardwood trees throughout the United States and Canada. Introduction of the exotic Asian gypsy moth into North America would be even more impactful, as Asian gypsy moth populations have wider host ranges, and are capable of naturally dispersing more rapidly due to female flight ability. To support early detection and exclusion of Asian gypsy moth, the U.S. Department of Agriculture (USDA) uses molecular techniques to screen moths trapped in North America for evidence of common Asian genotype. In order to strengthen U.S. domestic capacity to screen moths quickly and efficiently, we report a real-time PCR assay for this pest. A probe system using TaqMan 5' nuclease chemistry is reported for detection of an allele associated with common Asian gypsy moth genotypes. The targeted allele is located at the nuclear FS1 locus currently used by the USDA in conventional PCR tests to screen for evidence of Asian gypsy moth introductions or introgression. The diagnostic probe is successfully multiplexed with a conserved 18S probe system to detect reaction failure due to poor sample quality or quantity. The specificity, sensitivity, and repeatability of the FS1-18S multiplex real-time PCR assay were tested on laboratory-reared and field-collected moths to demonstrate diagnostic utility. Implications of the new assay as a screening tool for evidence of Asian gypsy moth introgression and introduction are discussed.