In mice there are two forms of the beta chain used in the IL3 receptor system, L L com and L L IL3 . L L com is used by the IL3, IL5 and GM-CSF receptors whereas L L IL3 is only used in the IL3 receptor. In this work an assay was developed to identify residues of L L IL3 that restrict IL5 activity. It was found that such residues reside within the 2nd CRM of the molecule. Furthermore, when residues in the L L IL3 BP P-CP P loop were replaced with L L com sequence a form of L L IL3 was produced that was able to respond to IL5. This region is also responsible for IL3 binding to L L IL3 in the absence of alpha chain. It is therefore an important structural motif of L L com and L L IL3 responsible for both ligand interaction and specificity.z 1999 Federation of European Biochemical Societies.
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